摘要
人干扰素α-2b的简易、高效生产工艺研究,包括高效率、小型化的发酵技术,不用单克隆抗体亲和层析的纯化工序,以及大肠杆菌表达的人干扰素α-2b包涵体蛋白的天然构型复性等问题,从10L基因工程大肠杆菌发酵液所得1000g菌体中得人干扰素α-2b约500mg,效价为1×10~8u/mg,设备投资少,生产成本低,产品表现了高纯度、高效价。适宜大量生产,为广大人民群众提供廉价高质量药品创造了条件。
The production process of interferon a-2b was established in our institute. The human interferon a-2b was expressed in E. coli by using P1. promoter, The production process consists of high cell density fermentation, refolding of the inclusion body, chromatography with Sephadex G-50 and DEAE, without using monocloning antibody affinity chromatography. After purification,about 500mg of interferon a-2b was obtained from 1kg wet cell produced by 10L fermentation broth. The biological activity shows 1 - 2x108IU/mg protein. This production process is very simple and convenient. It will save a lot of facility investment, space and labour, so that the cost of the product is rather low.
出处
《生物工程学报》
CAS
CSCD
北大核心
1996年第1期23-27,共5页
Chinese Journal of Biotechnology
