摘要
根据正交试验得出无花果曲霉原生质体形成的最佳条件，用１％的混合酶液（０．５％纤维素酶＋０．２５％蜗牛酶＋０．２５％溶菌酶）作用无花果曲霉菌体细胞，原生质体产量达３．２×１０７个·ｍｌ－１，渗透压稳定剂为０．６ｍｏｌ·Ｌ－１ＫＣｌ于０．２ｍｏｌ·Ｌ－１ＰＯ３＋４（ｐＨ５．８）中，酶解时间和酶解温度分别为３．０ｈ、３０℃．比较不同酶解时间、再生稳定剂和碳源等因素对原生质体再生的影响，可确定最佳再生条件，再生率达３０％以上．
Orthogonal experiments were designed to study the optimized conditions for protoplast formation of Aspergillus ficuum . A 1% mixed solution of enzymes (0.5% cellulase + 0.25% snail enzyme + 0.25% lysozyme) was used to treat the body cells of A.ficuum , and the yield of protoplast was up to 3.2 × 10 7 individuals·ml -1 . The optimum osmotic stabilizer was 0.6 mol·L -1 KCl containing 0.2 mol·L -1 PO 3+ 4 (pH 5.8), and the optimum duration and optimum temperature for enzymatic action was 3.0h and 30℃, respectively. The optimal conditions for protoplast regeneration were also obtatined, and the regeneration rate was over 30%.
出处
《应用生态学报》
CAS
CSCD
1998年第4期440-442,共3页
Chinese Journal of Applied Ecology
基金
国家"八五"科技攻关资助
关键词
无花果曲霉
原生质体形成与再生
Aspergillus ficuum ,Protoplast formation and regeneration.
