摘要
目的：为证明构成内毒素（ＬＰＳ）的主要核心部分类脂Ａ（ＬｉｐｉｄＡ）在ＬＰＳ直接损伤内皮细胞中所起的作用。方法：分离培养人肾小球内皮细胞（ＨＧＶＥＣ），把细胞分为正常对照组、ＬＰＳ时间梯度组、ＬＰＳ浓度梯度组和ＬｉｐｉｄＡ浓度梯度组。线粒体脱氢酶（Ｍｉｔｏｃｈｏｎｄｒｉａｄｅｈｙｄｒｏｇｅｎａｓｅ，ＭｔＤＨ）活性采用ＭＴＴ法测定。结果：时间梯度组：１μｇ／ｍｌＬＰＳ使内皮细胞线粒体脱氢酶活性呈进行性下降，在１２ｈ时比正常对照组下降显著（Ｐ＜０．０１）。浓度梯度组ＬＰＳ在０．９μｇ／ｍｌ使线粒体脱氢酶活性显著下降，而ＬｉｐｉｄＡ在０．６μｇ／ｍｌ处即可出现显著下降。结论：ＬＰＳ和ＬｉｐｉｄＡ均可直接损伤ＨＧＶＥＣ，使其线粒体脱氢酶活性下降，ＬｉｐｉｄＡ的损伤作用强于ＬＰＳ，证明ＬｉｐｉｄＡ在ＬＰＳ引起的内皮细胞直接损伤中起主要作用。
Objective: To investigate the injurious effects of LPS and lipid A on vascular endothelial cells (VECs). Methods: Cultured human glomerular vascular endothelial cells (HGVECs) were divided into the control group and the groups treated with LPS in different time durations, the groups treated with LPS in different concentrations and the groups treated with lipid A in different concentrations. The activity of mitochondrial dehydrogenase was measured with MTT. Results: LPS 1 μg/ml inhibited significantly the activity of mitochondrial dehydrogenase ( P <0.01). The inhibiting concentration was lower in lipid A (0.6 μg/ml) than in LPS (0.9 μg/ml). Conclusion: Both LPS and lipid A possess direct injurious effects on HGVECs and inhibit the activity of mitochondrial dehydrogenase. The inhibition is more powerful in lipid A than in LPS. The finding suggests that lipid A plays a major role in the injurious effects on VECs.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第4期253-255,共3页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
类脂A
内毒素
线粒体脱氢酶
烧伤
HGVEC
lipid A
LPS
human glomerular vascular endothelial cell
mitochondrial dehydrogenase
MTT
