摘要
研究了乳酸环丙沙星的极谱伏安行为。在ｐＨ＝８．３的０．０２ｍｌ／ＬＮＨ４Ｃ１－ＮＨ３Ｈ２Ｏ底液中，乳酸环丙沙星能产生１个灵敏的还原峰，峰电位为－１．６１Ⅴ，其导数峰高与浓度在３．９＊１０－８～１．８＊１０－ｍｏｌ／Ｌ和１．８＊１０－６～１．１＊１０－５ｍｏｌ／Ｌ范围内呈现良好的线性关系，相关系数ｒ＝０．９９９７，检测下限为２．５＊１０－８ｍｏｌ／Ｌ．用于乳酸环丙沙星注射液的测定，相对标准偏差为０．３８％～０．７２％，回收率为９９．２％～１００．５％。大多数金属离子和无机阴离子不干扰测定，此方法还可用于血清、血浆及尿样中痕量乳酸环丙沙星的测定，方法简便、快速、灵敏，用多种电化学手段证明乳酸环丙沙星的还原属于反应物吸附的不可逆过程．
The polarographic and voltammetric behavior of ciprofloxacin lactate (CFL) and its application were studied. In 0.02 mol/L NH4C1-NH3. H2O buffer solution (pH= 8.3),a well-defined reduction peak was yielded by CFL. The peak potential(Ep) is about -1. 61 V (vs-SCE).A linear relationship holds between the differential peak height and the concen tration of CFL in the range of 3.9×10-8～1.8×10-5 mol/L and 1.8 ×10-6～1. 1×10-5 mol/L, respectively, with r= 0. 999 7.The limiting detection is 2.5×10-8 mol/L. Most of metallic ions do not interfere the determination. This method is used to determine CFL in raw samples and injections with RSD 0.38%～0.72% and recoveries 99. 2%～101.4%, and can also be used to determine the trace CFL in serum, plasma and urine samples. It may provide a simple, rapid and sensitive method for determining clinical medicine and pharmacokinetics.The experimental results show that the reduction of CFL belongs to an irreversible process of reactant adsorption. The electrode reaction mechanism was preliminarily presented.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1996年第6期874-877,共4页
Chemical Journal of Chinese Universities
关键词
乳酸环丙沙星
示波极谱法
吸附波
Ciprofloxacin lactate, Single-sweep oscillopolarography, Polarographic behavior, Adsorptive wave
