摘要
目的：采用快速固相萃取反相高效液相色谱法测定全血环孢素Ａ。方法：全血用水溶血，用乙腈和水混合液沉淀蛋白后，用ＳＰＥ固相萃取柱萃取，５０％甲醇冲洗杂质，９５％甲醇洗脱，挥干，乙腈重组，正己烷进一步除去杂质，重组液上机。本法采用柱温为７０℃的Ｃ１８反相色谱柱，以乙腈∶甲醇∶水（３∶１∶１，Ｖ∶Ｖ）为流动相，检测波长２１０ｎｍ。结果：本法最低检出浓度为２０ｎｇ·ｍｌ－１。至少在４０～１５００ｎｇ·ｍｌ－１范围内呈线性。平均方法回收率１０２．９％，ＲＳＤ均在１０％以内。结论：本法灵敏，特异，既适合临床常规检测又适合ＣｓＡ的药动学研究。
OBJECTIVE :To develop a reversedphase liquid chromatographic method utilizing,a solidphase extraction procedure for the determination of cyclosporine A (CsA) in human whole blood. METHODS :The drug was extracted from whole blood by solidphase extraction,then separated isocratically using a C18 column and a mobile phase of acetonitrilemethanolwater (311).The column temperature was maintained at 70.The wavelength used for detection was 210nm. The cyclosporine D was used as interal standard. RESULTS :The lowest limit of detection of CsA was 20ngml-1.A linear relationship was good at least from 40ngml-1 to 1500ngml-1.The average recoveries of CsA were 102.9% for whole blood.RSD values of withinday and betweenday were all lower than 10%. CONCLUSIONS :The method is simple and accurate.It is suitable not only for routine determination of CsA but also for the study of pharmacokinetics of CsA.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1999年第5期275-277,共3页
Chinese Journal of Hospital Pharmacy
