摘要
目的：研究不同人群中红细胞ＣＲ１基因组密度多型性与红细胞免疫粘附肿瘤细胞能力的相关性。方法：采用ＰＣＲ加ＨｉｎｄⅢ酶切技术测定红细胞ＣＲ１基因组密度多型性（ＨＨ型、ＨＬ型、ＬＬ型），采用肿瘤红细胞花环试验测定红细胞免疫粘附肿瘤细胞的能力。结果：在不同人群中，ＨＨ型红细胞免疫粘附肿瘤细胞的能力明显大于ＨＬ型红细胞，而ＨＬ型红细胞免疫粘附肿瘤细胞的能力明显大于ＬＬ型红细胞。结论：红细胞免疫粘附肿瘤细胞的能力与ＣＲ１基因组密度多型性有关。
Objective: To study correlation between CR1 genomic density
polymorphism of erythrocytes and ability of erythrocytes adhering to tumour cells in variant
population(normal people, patient with HBV infection, with hepatocirrhosis and with
hepatocarcinoma). Methods: Genomic determination of the CR1 density polymorphism (HH
type, HL type, LL type) of erythrocytes was performed using polymerase chain reaction
amplification and Hind retriction enzyme digestion. Ability of erythrocytes adhering to tumour
cells using tumour erythrocyte rosetest. Results : In different population,ability of HH type
erythrocytes adhering to tumour cells was significantly higher than that of HL type erythrocytes.
Ability of HL type erythrocytes adhering to tumour cells was significantly higher than that of LL
type erythrocytes. In same CR1 genomic type population, ability of erythrocytes adhering to
tumour cells in normal people significantly higher than that in patients with HBV infection and
patients with hepatocirrhosis and patients with hepatocarcinoma. Conclusion: These results
indicate that activity of erythrocytes adhering to tumour cells may be related to CR1 genomic
density polymorphism type.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第5期327-329,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
关键词
受体
红细胞
CR1基因
肿瘤
免疫疗法
免疫粘附
receptors, erythrocytes
tumor cells adhesion
genomic
density polymorphism
