摘要
目的为了研究不同肝病患者人群中红细胞ｃｒ１基因组密度多态性与红细胞免疫粘附肿瘤细胞能力的相关性．方法乙型肝炎患者４８例，肝硬变４９例，肝细胞性肝癌９４例及正常对照８０例．采用ＰＣＲ加ＨｉｎｄⅢ酶切技术测定红细胞ｃｒ１基因组密度多态型（ＨＨ型、ＨＬ型、ＬＬ型），采用肿瘤红细胞花环试验测定红细胞免疫粘附肿瘤细胞的能力．结果在不同人群中，ＨＨ型红细胞免疫粘附肿瘤细胞的能力明显大于ＨＬ型红细胞，而ＨＬ型红细胞免疫粘附肿瘤细胞的能力明显大于ＬＬ型红细胞．在相同基因型人群中，正常人红细胞免疫粘附肿瘤细胞能力显著高于乙型肝炎、肝硬变和肝癌患者．结论红细胞免疫粘附肿瘤细胞的能力与ｃｒ１基因组密度多态型别有关，同时也与后天获得性影响因素有关．
AIM To study the relationship between cr 1 genomic density polymorphism on erythorocytes and ability of erythrocytes adhering tumor cells in various populations, with HBV infection, liver cirrhosis and liver cancer. METHODS Genomic determination of the cr 1 density polymorphism (HH type, HL type, LL type) on erythrocytes was measured with polymerase chain reaction amplification and Hind Ⅲ retriction enzyme digestion, and the ability of erythrocytes adhering tumor cells was studied using tumor erythrocyte rose test in 46 patients with hepatitis B, 49 patients with liver cirrhosis, 94 patients with hepatocellular carcinoma and 80 normal controls. RESULTS The ability of HH type erythrocyte adhering tumor cells was significantly higher than that in HL type erythrocytes. The ability of HL type erythrocytes adhering tumor cells was significantly higher than that in LL type erythrocytes. In the same cr 1 genomic type population, the ability of erythrocytes adhering tumor cells of normal people was significantly higher than that of patients with HBV infection and patients with livercirrhosis and patients with liver cancer. CONCLUSION The activity of erythrocytes adhering tumour cells is related to cr 1 genomic density polymorphism type to acquired influence factor.
基金
国家自然科学基金
关键词
红细胞
cr1基因
肝硬化
肝肿瘤
liver diseases
erythrocytes cr 1 gene
hepatitis B
liver cirrhosis
liver neoplasms
