摘要
背景:现有各类组织工程皮肤面临着共同的难题,突出表现在没有完整的皮肤功能,尤其是缺乏皮肤附属物如毛囊、汗腺、皮脂腺等。如何培养具有皮肤附属物的"功能性组织工程皮肤"即完整的皮肤器官,成为皮肤组织工程迫在眉睫的研究方向。目的:文章通过综述皮肤附属物相关干细胞的分离与培养以及向皮肤附属物诱导分化的研究进展,为解决当前人工复合皮肤中难以形成皮肤附属物的难题提供帮助。方法:应用计算机检索Pubmed数据库(1999-01/2009-12),以"skin tiss ue engineering,skin appendages"为检索词;应用计算机检索中国期刊全文数据库(2004-01/2009-12),以"皮肤组织工程、皮肤附属物、毛囊、汗腺、皮脂腺"为检索词。纳入标准:文章所述内容应与利用组织工程技术使干细胞分化、增殖形成毛囊、汗腺、皮脂腺等皮肤附属物研究相关。排除标准:重复研究或荟萃分析类研究。结果与结论:计算机初检得到97篇文献,排除因研究目的与此文无关的35篇,内容重复性的研究26篇,共保留36篇文献进行综述。人体多种细胞都有向皮肤附属物分化增殖的能力,进一步阐明胚胎发育的机制,深入研究干细胞定向分化的调控机制,在皮肤修复的同时能够诱导毛囊、皮脂腺和汗腺的发生,无疑是构建理想组织工程皮肤的重要途径。将生长因子、干细胞生物技术与生物材料工程学相结合,才可能研制成功真正意义上的皮肤永久替代物,但体外研制成功的皮肤永久替代物应用于人体的安全性及效果还有待进一步分析。
BACKGROUND：All types of tissue-engineered skins have a common challenge,namely,the absence of complete skin function,especially lacking of skin appendages（hair follicles,sweat glands,sebaceous glands,etc.）.How to develop the ＂functional tissue-engineered skin＂,that is a complete skin and organ,has become an imminent research direction.OBJECTIVE：To provide help for solving the problem that it is hard to form skin appendages in artificial composite skins by reviewing progress of isolating or culturing skin appendage-related stem cells and inducing differentiation of skin appendages.METHODS：A computer online search using Key words of ＂skin tissue engineering,skin appendages＂ was performed to search databases of PubMed and CNKI.Inclusive criteria：Articles related to differentiation and proliferation of stem cells into hair follicles,sweat glands,sebaceous glands and other skin appendage using tissue engineering technology.Exclusive criteria：repetitive research or Meta analysis. RESULTS AND CONCLUSION：A total of 97 literatures were searched by computer,and 36 papers were further analyzed after excluding 35 unrelated articles and 26 repetitive research.A variety of cells in the human body has the ability to differentiate into skin appendages.Further clarifying the mechanism of embryonic development,in-depth study of stem cells differentiation regulation mechanism,combining growth factors,stem cell biotechnology and bio-materials engineering may develop a real sense of the permanent skin substitute.However,the safety and effect of permanent skin substitute need to be further studied.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第33期6210-6213,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
