摘要
目的观察透明质酸（ｈｙａｌｕｒｏｎｉｃａｃｉｄ，ＨＡ）刺激活性物（ＨＡ－ｓｔｉｍｕｌａｔｉｎｇａｃｔｉｖｉｔｙ，ＨＡＳＡ）对培养的人视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉａｌ，ＲＰＥ）细胞的作用。方法将不同质量浓度的ＨＡＳＡ加入ＲＰＥ细胞培养液，采用活细胞计数、四甲基偶氮唑盐（ｔｅｔｒａｚｏｌｉｕｍ，ＭＴＴ）比色法及氚标胸腺嘧啶核苷（３Ｈ－ｔｈｙｍｉｄｉｎｅ，３Ｈ－ＴｄＲ）掺入法检测细胞活力，用流式细胞仪（ｆｌｏｗｃｙｔｏｍｅｔｒｙ，ＦＣＭ）分析ＲＰＥ细胞周期。结果ＨＡＳＡ在１２．５～２００．０μｇ／ｍｌ范围及４８小时内对人ＲＰＥ细胞的抑制存在剂量－效应及时间－效应关系。最大细胞抑制率约４８．０％。ＦＣＭ显示ＨＡＳＡ作用后Ｇ１期细胞数较对照组增加７．２％，Ｓ期减少９．７％。结论ＨＡＳＡ在一定时间和剂量范围内对人ＲＰＥ细胞有抑制作用。
Purpose To study inhibition effects of retinal pigment epithelial (RPE) cellsby hyaluronic acid
stimulating activity(HASA).Methods The cultured human RPE cellsadded with a series of
HASA was measured with cell counting,tetrazolium(MTT)colorimetric assay and tritiumlabelled
thymidine deoxyribose(3HTdR)incorporation assay.Flow cytometry(FCM)analysis was used to
examine RPE cells cycles. ResultsHASA at concentrations of 12.5200 g/ml and within 48
hours inhibited RPE cells proliferation with a dosedependant and timedependant manners.The
maximal inhibition rate of RPE cells by HASF was about 48.0%.FCM revealed that the cells in
G1 phase increased 7.2% and cells in S phase decreased 9.7%,compared to controls.
Conclusion HASA at a certain dose range and period can inhibit RPE cells proliferation.
出处
《中华眼底病杂志》
CAS
CSCD
北大核心
1999年第2期72-74,共3页
Chinese Journal of Ocular Fundus Diseases
基金
国家自然科学基金
关键词
细胞培养
透明质酸
玻璃体
视网膜病变
色素上皮
Pigment epitheliumofeyeCell,culturedHyaluronic acid/analysisFlow
cytometryvitreoretionopathy,proliferative
