摘要
对雄性大鼠进行铬染毒，剂量为每ｋｇ体重２５５ｍｇＣｒ６＋，每天１次，连续染毒７ｄ，停止染毒后继续观察７ｄ。结果染毒１ｄ后，大鼠肝肾组织中的低分子络结合物ＨＭＷＣｒ，ＬＭＷＣｒ及ＭＭＷＣｒ较对照组显著升高，并随染毒时间的延长而不断升高；对照组中未检出ＭＭＷＣｒ，可作为铬中毒的特异观察指标。采用葡聚糖凝胶Ｇ５０柱层析法分离低分子蛋白效果好。
Male Wistar rats were injected intraperitoneally with potassium dichromate(2.55mg Cr 6+ per kg body weight) for 7 days, and were observed continously for 7 days after stopping Cr injection. The results were that concentrations of chromium binding substances (HMW Cr, LMW Cr, and MMW Cr) increased significantly with the extension of Cr injection time; MMW Cr was not found in the control group. MMW Cr may be a valuable indicator in diagnosing chromium toxication. It was efficient to use Sephadex G 50 column chromatography and ultra violet absorption spectrometry atomic absorption spectrophotometry in isolation, and then it was followed by quantitative analysis of chromium binding substances.
出处
《湖南医科大学学报》
CSCD
1999年第3期222-224,共3页
Bulletin of Hunan Medical University
基金
国家自然科学基金
关键词
铬中毒
动物模型
低分子络结合物
chromium
poisoning
spectrophotometry,atomic absorption
disease models animals
rats
