摘要
目的探讨抗精子抗体在实验性精索静脉曲张大鼠中的表达及其对精子质量的影响。方法将30只雄性SD大鼠随机分为三组:对照组(n=10)、模型组(n=10)和治疗组(n=10)。模型组和治疗组均进行左肾静脉缩窄建立左侧精索静脉曲张模型;对照组只进行左肾静脉分离,不缩窄左肾静脉。利用全自动精液分析系统分析大鼠附睾尾部精子质量;利用酶联免疫法(ELISA)检测各组血清及附睾腔液中抗精子抗体的吸光度值。结果正常组和治疗组精子密度显著高于模型组,差异具有统计学意义(P<0.05);三组间精子活动率、血清和附睾腔液抗精子抗体的吸光度值差异无统计学意义(P>0.05)。精子密度、活动率与血清和附睾腔液抗精子抗体OD值之间未见线性相关(P>0.05)。结论抗精子抗体在VC大鼠血清和附睾腔液中表达量低,且对精子质量无影响,提示抗精子抗体与精索静脉曲张关系不大。
Objective To investigate the level of anti-sperm antibody（AsAb）in experimental varicocele rats and its effect on sperm quality.Methods Thirty rats were randomized into three groups：control group（n=10）,model group（n=10）and treatment group（n=10）.Experimental varicocele model was established in model group and treatment group by partial ligation of left renal vein.Computer-aided semen analysis system was used to detect the sperm density and motility.The levels of AsAb in serum and epididymal lumen fluid were measured by enzyme-linked immunosorbent assay（ELISA）.Results The sperm density in model group were significantly lower than in control group and treatment group（P〈0.05）.The OD value of AsAb,and the sperm motility among three groups showed no statistical difference（P〉0.05）.No correlation of sperm density and motility with the level of AsAb was found（P〉0.05）.Conclusion AsAb has low level in rats with varicocele,but can not affect the sperm quality,suggesting that there may be no relationship between AsAb and VC.
出处
《山西医科大学学报》
CAS
2010年第9期789-791,共3页
Journal of Shanxi Medical University
基金
山西省自然科学基金资助项目(2009011057-3)
