摘要
目的:探讨caspase-3在精索静脉曲张大鼠生精细胞中的表达及其与生精细胞凋亡的关系。方法:雄性SD大鼠24只,随机分为假手术组(SOG)、30 d术后组(PG1)、60 d术后组(PG2),每组8只。建立左精索静脉曲张模型,TUNEL法检测生精细胞凋亡,免疫组化SABC法检测caspase-3表达。结果:SOG、PG1、PG2大鼠左、右侧睾丸每生精小管截面caspase-3阳性细胞数分别为0.117 5±0.012 9、0.246 3±0.042 1、0.293 8±0.051 1及0.165 0±0.019 2、0.253 8±0.021 9、0.277 5±0.034 3。与SOG相比,PG1和PG2组大鼠双侧睾丸生精细胞caspase-3表达均增加,差异有显著性(P=0.011 5及P=0.014 4)。结论:精索静脉曲张可能是大鼠生精细胞caspase-3表达增加且生精细胞凋亡增多的机制之一。
Objective ： To explore the relationship between the expression of caspase-3 in testicular germ cells of rats with experimental left varicocele （ELV） and apoptosis of germ cells. Methods ： Twenty-four male SD rats were randomly divided into three groups with eight animals each： sham-operation group （ SOG）, 30-day post-operation group （ PG1 ） and 60-day psot-operation group （PG2）. ELV model was established by the partial ligation of the left renal vein. To detect apoptosis of germ cells and expression of caspase-3, TUNEL assay and immunohistochemistry（SABC）were used respectively. Results： The number of caspase-3 positive germ cells per tubular cross section in left and fight testes of rats in SOG, PG1, PG2 were 0. 117 5 ± 0.012 9, 0.246 3 ± 0.042 1, 0.293 8 ± 0.051 1 and 0. 165 0 ± 0.019 2, 0.253 8 ± 0.021 9, 0.277 5 ± 0.034 3, respectively. Compared with SOG, the expression of caspase-3 in bilateral testes of rats in PGI and PG2 were increased, and the differences were statistically significant（P =0.011 5 and P =0. 014 4）. Conclusion： Expression of caspase-3 protein increased in germ cells of rats with ELV, which may be one of the molecular mechanisms related to excessive testicular germ cell apoptosis.
出处
《中华男科学杂志》
CAS
CSCD
2006年第1期53-56,共4页
National Journal of Andrology
