摘要
[目的]建立从锁阳中富集儿茶素的方法,并对儿茶素含量进行测定。[方法]用50%乙醇对锁阳进行超声提取,提取物经大孔吸附树脂对儿茶素进行富集,并对儿茶素富集部位进行含量测定。HPLC工作条件为:色谱柱C18(250.0mm×4.6mm,5μm);流动相为乙腈∶0.2%乙酸15∶85(V/V);柱温为室温;流速1ml/min,检测波长280nm。[结果]锁阳中的儿茶素主要集中在35%乙醇洗脱部分;阴性对照品与样品分离良好,儿茶素的线性范围为0.005～0.040mg/ml(r=0.9999),回收率为98%,RSD为0.658%。[结论]用大孔吸附树脂可从锁阳提取物中高效地分离纯化富集儿茶素;HPLC检测方法可行,测定误差小,结果较准确,重现性好。
[Objective] To study the isolation and purification methods of catechin of Cynomorium songaricum Rupr.and establish a HPLC method for the determination of catechin.[Methods]The dried Cynomorium songaricum Rupr.powder was extracted with 50% EtOH and purified via column chromatography on macroporous adsorbent resin.Using the reversed-phase HPLC determine the content of catechin of above fraction.The qualification of HPLC：C18column（250.0 mm×4.6 mm,5 μm）,with mobile phase consisted of acetonitrile：0.2%acetic acid（V/V）（15∶ 85）.The column temperature was room temperature.The flow rate was 1 ml/min and the detection wavelength was 280 nm.[Results]Catechin is concentrated the 35% EtOH eluting fraction.The sample and the negative control were separated well by HPLC.The linear ranges of catechin was 0.005-0.040 mg/ml（r=0.999 9）.The average recoveries of catechin was 98%（RSD=0.658%）.[Conclusion] This method is simple and rapid to isolate and purify the catechin from Cynomorium songaricum Rupr.and,the feasible quantitative analysis method with small determinate error,accurate result and good reproducibility.
出处
《安徽农业科学》
CAS
北大核心
2010年第20期10644-10645,共2页
Journal of Anhui Agricultural Sciences
基金
北京市教委科技计划项目(KM200811417002)
关键词
锁阳
儿茶素
大孔吸附树脂
高效液相色谱
Cynomorium songaricum Rupr.
Catechin
Macroporous adsorbent resin
HPLC
