摘要
[目的]研究利用莱氏野村菌对棉铃虫进行生物防治的效果。[方法]采用浸渍接种法,用莱氏野村菌MZ060806-XW分生孢子的5个浓度(3.5×104～3.5×108个/ml)对棉铃虫2龄和3龄幼虫进行致病性研究。[结果]在莱氏野村菌MZ060806-XW分生孢子3.5×108个/ml浓度下;第8天2龄和3龄的幼虫死亡率最高分别达84.68%和82.23%;第8天,2龄和3龄幼虫的致死中浓度(LC50)分别为3.975×104和7.794×104个/ml。在3.5×104～3.5×108个/ml的5个浓度处理下,2龄幼虫的致死中时间(LT50)依次为9.774、6.322、5.415、4.559和4.051d,3龄幼虫的LT50分别为9.462、6.842、5.365、4.636和4.177d。随着孢子浓度的增加,LT50逐渐缩短,虫口的死亡率逐步增加;随着施药时间的推移,LC50逐渐减小。[结论]利用莱氏野村菌MZ060806-XW菌株对棉龄虫2龄幼虫的防治效果高于3龄幼虫。莱氏野村菌用于害虫的生物防治具有开发利用价值。
[Objective] The propose was to use the Nomuraea rileyi to make the biological control of Helicoverpa armigera.[Method] The pathogenicity research was conducted on the 2nd and 3nd instar larvae of H.armigera with conidia of N.rileyi MZ060806-XW under 5 different concn.of 3.5×104-3.5×108 spores/m inoculated by dipping method.[Result] The pathogenicity of conidia of N.rileyi MZ060806-XW to the mortality rate of the 2nd and the 3nd instar larvae was 84.68% and 82.23% resp.under concn.of 3.5×108 spores/ml on 8th day.LC50 of the 2nd and 3nd instar larvae were 3.975×104 and 7.794×104 spores/ml on 8th day.Under 5 concn.of 3.5×104-3.5×108 spores/ml,the LT50 of the 2nd instar larvae were 9.774,6.322,5.415,4.559 and 4.051 d resp.and that of the 3nd instar larvae was respectively 9.462,6.842,5.365,4.636 and 4.177 d.With the increase of spore concn.,the LC50 time was gradually shortened and the insect mortality was gradually increased;with the process of the drug application time,the LC50 concn.was gradually decreased.[Conclusion] The control effect of N.rileyi MZ060806-XW on the 2nd instar larvae of H.armigera was higher than that on 3nd instar larvae.N.rileyi used in the biological control of the pests had the value of development and utilization.
出处
《安徽农业科学》
CAS
北大核心
2010年第22期11918-11919,11959,共3页
Journal of Anhui Agricultural Sciences
基金
红河学院校级项目(XJ1Y0804)
