摘要
为实现苜蓿（Medicago sativa）的遗传改良,建立稳定、高效的离体培养再生体系,以甘农1号、巨人、WL324和三得利4个苜蓿品种为试验材料,以下胚轴为外植体,MS、UM、改良HS、改良UM为基本培养基,分别添加2,4-D、KT和水解酪蛋白诱导愈伤组织,通过体细胞胚发生途径建立再生体系,研究光照对苜蓿愈伤组织诱导及愈伤组织形态对体细胞胚发生的影响。结果表明,光照对愈伤组织诱导率差异不显著,均为100%;光暗交替条件下诱导的愈伤组织褐化率高、生长更快、更旺盛,颜色稍显绿或黄绿相间。在不同光照条件下4个苜蓿品种诱导的愈伤组织,以甘农1号的体细胞胚发生率最高,为20.83%~56.25%,而且光暗交替条件下诱导的愈伤组织更易形成体细胞胚;不同培养基中,UM＋0.1 mg/L NAA＋0.5 mg/L KT下体细胞胚发生率最高,为56.25%。
In order to achieve the genetic improvement of alfalfa and establish the high frequency and stable regeneration system,the hypocotyls of 4 alfalfa cultivars（Medicago varia cv.Gannong No.1,M.sativa cv.Ameristand,M.sativa cv.WL324 and M.sativa cv.Sanditi） were cultured on 4 basal medium（MS,UM,modified HS and modified UM） containing 2,4-D,KT and casein acid hydrolysate respectively.And the effect of light on callus induction and somatic embryo formation was studied.The result indicated that light showed no influence on callus induction and the callus rate was 100%.Callus induced under the light-dark alternation showed higher browning rate but rapid growth and more active,and showed light green or alternation of yellow and green.Callus induced from Gannong No.1 showed the highest somatic embryo developing rate which ranged from 20.83% to 56.25%.And it was easier to form somatic embryo under the light-dark alternation.The different medium were used to induce somatic embryo,UM ＋ 0.1 mg/L NAA ＋ 0.5 mg/L KT was the optimum medium and the rate of somatic embryogenesis was 56.25%.
出处
《草业科学》
CAS
CSCD
北大核心
2010年第9期109-114,共6页
Pratacultural Science
基金
国家"十一五"支持项目"牧草倍性育种技术研究"(2008BADB3B03)
山东省优秀中青年科学家科研奖励基金(BS2009SW010)
山东省农业良种工程牧草项目(2008LZ019-01
2008LZ019-04)
