摘要
目的探讨氧自由基对培养犬呼吸道上皮细胞合成释放小分子活性物质一氧化氮（ＮＯ），内皮素１（ＥＴ１），前列腺素Ｅ２（ＰＧＥ２］）和血栓烷素（ＴＸＡ２）的影响。方法培养的犬呼吸道上皮细胞与黄嘌呤（２６．３μｍｏｌ·Ｌ１）及黄嘌呤氧化酶（１７ｕ·Ｌ１）孵育４ｈ后，应用酶联免疫、放射免疫及Ｇｒｉｅｓ还原法，测定条件培养液中ＮＯ，ＥＴ１，ＰＧＥ２和ＴＸＡ２含量。结果氧自由基使犬呼吸道上皮细胞ＥＴ１和ＴＸＡ２释放量分别增加１２倍和１０倍；ＮＯ释放量减少６０％；同时使ＰＧＥ２的基础分泌量，组织胺刺激分泌量和花生四烯酸刺激分泌量均减少约７３％～８５％。结论氧自由基引起支气管上皮细胞参与调节支气管平滑肌舒缩的小分子活性物质分泌紊乱。
Objective To investigate the effects of oxygen free radicals on release of enogenous active molecules of cultured canine airway epithelial cells(CAEC),such as nitric oxide (NO),endotheline 1(ET 1),prostaglandin E 2(PGE 2) and theromboxane(TXA 2).Method Cultured CAEC were incubated with xanthine(26.3 μmol·L 1 )plus xanthine oxidase (17u·L 1 )(X XO)for 4 hours.The contents of NO,ET 1,PGE 2 and TXA 2 were measured by means of Griess assay,immunoradiation assay and ELISA,respectively.Results Treatment of X XO increased the release of ET 1 and TXA 2 by 12 and 10 folds, respectively. However,the release of NO and PGE 2 from CAEC were significanty reduced 60% and 73%～85% by X XO. Superoxiduse dismutase (100 u·L 1 )and catalase (200 u·L 1 )could block the effects of X+XO.Conclusion Oxygen free radicals leads disordered release of NO,ET 1,PGE 2 and TXA 2 airway epithelial cells,which is involved in the constringency of bronchus smooth muscle.
基金
国家自然科学基金
湖南省自然科学基金
关键词
氧自由基
呼吸道上皮
内皮素
血栓烷素
一氧化氮
oxygen free radicals
airway epithelial cells
endothelin
thromoboxane
nitric oxide
prostaglandin E 2
