摘要
目的研究念珠菌药敏试验的最佳条件，并测定５种抗真菌药物对７８株念珠菌的敏感性。方法采用稀释法测定最小抑菌浓度，并计算５０％菌株的最小抑菌浓度（ＭＩＣ５０）和９０％菌株的最小抑菌浓度（ＭＩＣ５０）。结果药敏测定条件以１０ｇ／Ｌ蛋白胨和２０ｇ／Ｌ葡萄糖组成的沙保弱培养基、ｐＮ７．０和接种菌量为１０７～１０８ＣＦＵ／ｍｌ为最好。两性霉素Ｂ、５－氟胞嘧啶、酮康唑和克霉唑ＭＩＣ５０分别为０．７８，１２．５，１２．５，６．２５μｇ／ｍｌ；ＭＩＣ９０分别为１．５６，２５，＞２５，２５μｇ／ｍｌ。氟康唑ＭＩＣ５０为２５μｇ／ｍｌ。除两性霉素Ｂ外，其余４种抗真菌药物对白色念珠菌的作用强于热带念珠菌。结论念珠菌药敏试验对选择有效抗真菌药物及研究耐药株的发生机制具有现实意义。
The best conditions of susceptibility testing of the Candida spp.were studied and five antimycotics were tested for susceptibility against 78 Candida spp.Methods MIC,including MIC50 and NIC90,was determined with broth dilution method.Results The best results of susceptibility testing were found in the Sabouraud media with 10 g/L peptone,20 g/L glucose,PH 7.0 and the inoculum concentration of 107108 CFU/ml.The MIC50 of amphotericin B,5-fluorocytosine,nizoral and kemazole were 0.78,12.5,12.5,6.25μg/ml,respectively,the MIC90 of these drugs were 1.56,25>25,25μg/ml,respectively.The MIC50 of fluconazole was 25μg/ml. Besides amphotericin B,other four drugs had better activity against Candida albicans than Candida tropicalis.Conclusion The susceptibility testing of the Candida spp.has practical significance in selecting the effective antimycotics and studying the developing mechanism of resistance isolates.
