摘要
本研究以天然甲壳素为原料,通过简单的化学、物理处理,制得一种用于人工肝的吸附解毒剂。经毒理学试验。体外静态测试和两种模型动物实验表明:该吸附解毒剂具有良好的生物相容性和血液相容性,对结合型胆红素和芳杂环氨基酸有较强的吸附能力。在研究黄疸模型动物实验中,实验犬经该吸附解毒剂进行灌流抢救后,在未作复原手术情况下,均能存活下来,且活动正常,未发现不良后遗症及其它不良反应。
A new adsorptive antidote was prepared from a natural chitin raw material by simple chemical and physical treatment.A static test showed that 1g of this antidote could decrease bilirubin in a 10ml plasma with a concentration of 50 units to 20-25 units within 2 hours and the amount adsorbed was 0.25-0.30 mg/g. As for endotoxin, it decreased from 54 PG/ml to 4-5 PG/ml and the amount adsorbed 500-510 PG/g. The amount of some hetero-and aromatic aminoacids adsorbed from complex amino acids were as follows: Phenylalanine 1.52mg/g; tyrosine 1.20mg/g histidine 1.31mg/g. Fulling 68-70 g of this adsorbent into an articiifal liver device, we performed a blood perfusion for 2 hours experimental dogs of icterus model weighing 15-18 kg. The contents of bilirubin and ghtamic pyruvic transaminase in the blood before and after perfusion decreased respectively by 53-63% and 42-48%, and heterocyclic and aromatic amino acids decreased respectively by: Phe. 47.3-49.1%, Tyr. 32.3-34.8%; His. 29.0-40.0%. The experimental dogs recovered normal activities and appetite soon and survived after perfusion. An experimental female dog even mated and got pregnant after perfusion. Nevertheless, experimental dogs of the same model without perfusion treatment would die within several days. In conclusion, Chitin has a good biological compatibility and a relatively strong adsorbability to bilirubin, endotoxin, hereto-and aromatic amino acids.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
1990年第2期131-136,共6页
Journal of Biomedical Engineering
