摘要
目的:研究子宫内膜癌(endometrial carcinoma,EC)组织中癌基因C-erbB-2、Ki-67的表达及血清肿瘤标志物CA125水平及三者与临床病理特征之间的关系。方法:收集手术切除标本EC组织48例及内膜对照组织30例,用免疫组化法检测C-erbB-2、Ki-67的表达;对所有病例术前均采用免疫放射分析测定血清CA125水平。结果:30例对照组织中C-erbB-2表达、Ki-67表达、血清CA125均阴性(0/30),而48例EC组织中三者阳性率分别为64.58%(31/48)、77.08%(37/48)、29.16%(14/48),与对照组比较差异显著(P<0.01)。C-erbB-2、Ki-67表达的阳性率以及血清CA125阳性率均与EC手术病理分期、组织病理分级有关(P<0.05),Ki-67表达、血清CA125水平与宫外淋巴结转移有关(P<0.05;P<0.01),三者与肿瘤浸润深度均有关系(P<0.05)。Spearman相关分析结果显示,C-erbB-2、Ki-67与CA125之间呈正相关关系(rs分别为0.284、0.346,P<0.05)。结论:C-erbB-2表达、Ki-67表达及血清CA125的阳性率反映了肿瘤不同阶段中各基因及肿瘤标志物的不同表现,联检对指导术后治疗具有一定的意义。
Objective To study the changes of expressions of C-erbB-2 and Ki-67 oncogenes in tumor tissue and serum levels of CA125 in patients with endometrial cancer of different stages.Methods Tissue expressions of C-erbB-2 and Ki-67 were examined with immuno-histochemistry in 48 surgical speciments of endometrial cancer and 30 specimens of non-cancerous hysterectomy.Serum CA125 levels were also determined with IRMA in these patients.Results Both the tissue expressions of C-erbB-2 and Ki-67 as wellas serum CA125 levels were negative in all the 30 patients with hysterectomy for benign disorders.Positive rate of tissue C-erbB-2,Ki-67 and serum CA125 level was 64.58%（31/48）,77.08（37/48）and 29.16%（14/48）respectively in the patients with endometrial cancer.Expressions of C-erbB-2 and Ki-67 were significantly higher in patients with more advanced diseases（lower grade differentiation,deeper penetration,positive lymph node metastasis）than those in patients with less advanced diseases（P〈0.05~0.01）.The same was true for the serum CA125 level.These three markers were mutually positively correlated.Conclusion Combined determination of tumor tissue C-erbB-2 and Ki67 expressions as well as serum CA125 in patients with endometrial cancer could reflect the seriousness of the disease and might be useful for outcome prediction.
出处
《放射免疫学杂志》
CAS
2010年第4期419-421,共3页
Journal of Radioimmanology
