摘要
目的：研究雌二醇（E2）对高糖（Glu，30mmol／L）环境中内皮细胞系EVC-304的凋亡及其膜型基质金属蛋白酶-1（MT1-MMP）mRNA表达的影响．方法：用E2干预高糖环境中的ECV-304，用四唑盐比色试验（MTT）检测高糖和E2对ECV-304增殖的影响；用流式细胞术（flowcytometry，FCM）和细胞基因组DNALadder法检测高糖和E2干预后ECV-304凋亡；用半定量RT—PCR法检测高糖和E2干预后ECV-304MT1-MMPmRNA表达．结果：高糖可以促进EVC-304凋亡（P〈0．01），也可以降低MT1-MMP的表达（P〈0．01）；E2在浓度为0．01～0．10mmol／L时，可以拮抗高糖的促凋亡效应（P〈0．01），也可恢复性上调MT1-MMP的表达（P〈0．05）．E2的这种效应不随时间变化而变化（P〈0．05）．结论：高糖对内皮细胞具有毒性作用，E2对内皮细胞具有保护作用；E2还可以上调高糖环境中内皮细胞MT1-MMP的表达．
AIM： To explore the effect of 17-β estradiol （ E2 ）on apoptosis and membrane-type matrix metalloproteinase 1 （ MT1- MMP） mRNA expression in endothelial cell line EVC-304 under the high glucose condition 30 mmoL/L. METHODS： The proliferation of EVC-304, which is treated by high glucose（30 mmoL/L） and/or E, of different concentrations respectively, was measured by MTI＂ assays. Then the apoptosis of ECV-304 was detected by flow cytometry （ FCM ） and DNA ladder assays. Finally, MT1 - MMP mRNA expression was measured by semi-quantitative RTPCR. RESULTS： After ECV-304 had been treated by high glucose（30 mmol/L） , its proliferation and mRNA expression of MT1-MMP was inhibited significantly（ P 〈 0.01 ）. However, the tendency fall back after E2 had been administrated （ P 〈 0.01 ）. Furthermore, this effect of E2 reached a peak at its concentration from 0.01 to 0. 10 mmoL/L. The apoptosis was only detected in cells which had been administrated by high glucose and E2 at low （0.001 nmol/L） and high （ 1.0 nmol/L） concentration by DNA Ladder assays. CONCLUSION： High glucose has a strong toxicity on vascular endothelial cells. Moderate level of E2 has a protective effect on vascular endothelial cells. E2 can enhance MT1- MMP expression of endothelial cells under high glucose condition.
出处
《第四军医大学学报》
北大核心
2006年第18期1700-1703,共4页
Journal of the Fourth Military Medical University
关键词
内皮/细胞学
间质胶原酶
雌二醇
高糖
逆转录聚合酶链反应
细胞凋亡
endothelium/cytology
interstitial collagenase
estradiol
high glucose
reverse transcriptasepolymerase chain reaction
apoptosis
