摘要
研究了神经节苷脂ＧＭ_３参入肌质网膜后Ｃａ^（２＋）－ＡＴＰ酶活力的变化，结果表明：ＧＭ_３参入肌质网膜后，对肌质网Ｃａ^（２＋）ＡＴＰ酶活性（ＡＴＰ水解活力与转运活力）有明显的激活作用．当参入的ＧＭ_３浓度为８μｍｏｌ／Ｌ、参入时间为１２０ｍｉｎ、温度为３０℃时，对Ｃａ^（２＋）－ＡＴＰ酶的激活作用最大．
Ganglioside GM_3 from canine erythrocytes was incubated with rabbit sarcoplasmic reticulum (SR) vesicles to observe its effect on SR Ca￣(2+)-ATPase. The results showed that GM_3 could incorporate into the membrane of SR and significantly activated SR Ca￣(2+)-ATPase (including ATP hydrolysis activity and Ca￣(2+)-uptake activity). Such an activation was dependent on GM_3 concentration,and incubation temperature and time. The optimum activation was observed when 8μmol/L GM_3 was incubated with SR vesicles for 120 min at 30℃.
基金
生物大分子国家重点实验室基金
