摘要
对灰树花进行了液体深层培养试验，结果表明：液体培养基碳源以豆粉＋麸皮、玉米粉＋麸皮和氮源以蛋白胨、牛肉膏等较适宜菌丝生长的物质，确定了灰树花深层培养的液体培养基配方；并研究了培养条件对菌丝生物量的影响，得出了培养液ｐＨ在５３０～６００和通气量为１：（１００～１４０）的条件下。
By submerged culture experiment, Grifola frondosus ,the results showed that the optimum carbon source are been powder+bran and corn flour+bran,the optimum nitrogen source are peptone and beef cream,the optimum inorganic salts are KH 2PO 4、CaCO 3、MgSO 4、FeSO 4、ZnSO 4、and CuSO 4,of which are befitted to mycelia growing.The research on culture conditions of Grifola frondosus were carried out by submerged culture.At pH 5.30～6.00 and the flow of air compress at 1:(1.00～1.40) the biomass of mycelia reached the highest level.Thereby,the ingredients of liquid media and culture conditions of Grifola frondosus were determined and the base was settled for further research in future.
出处
《生物工程学报》
CAS
CSCD
北大核心
1999年第3期378-382,共5页
Chinese Journal of Biotechnology
关键词
灰树花
深层培养
碳源
氮源
Grifola frondosus ,submerged culture,carbon source,nitrogen source
