摘要
目的:探讨伊文思蓝染色技术快速鉴定新型隐球菌在临床检验医学诊断工作中的应用。方法:配制伊文思蓝染色液,评估伊文思蓝染色液的稳定性及其;用伊文思蓝染色液对新型隐球菌、白色念珠菌等常见真菌及白细胞染色,综合评价其染色效果,进一步比较伊文思蓝染色液、阿利新蓝染色液、墨汁对新型隐球菌的染色效果。结果:新型隐球菌被伊文思蓝染色液染成蓝色或深蓝色,白细胞及其他常见真菌不着色,易于将新型隐球菌与白细胞及其他常见真菌区分;伊文思蓝染色液稳定性好,染色效果稳定,优于阿利新蓝染色和墨汁染色,伊文思蓝染色能在计数板中计数新型隐球菌,阿利新蓝染色易凝集对计数有一定的影响,而墨汁染色则无法计数。结论:伊文思蓝染色技术快速鉴定新型隐球菌设备要求简单、方法快速敏感,有很好的和稳定性,适用各类医院推广应用。
Objective：To study rapid identification of Cryptococcus neoformans by Evans blue staining technology in clinical laboratory diagnosis.Methods： Prepare Evans blue staining reagent,to evaluate the stability and specificity of the reagent,using Evans blue staining reagent to stain fungus like Cryptococcus neoformans,Candida albicans and white blood cells,Comprehensive evaluating its stain effects,further comparing stain effects of Cryptococcus neoformans between Evans blue stain,Alcian blue stain and ink stain.Results： The Evans blue stain of Cryptococcus neoformans cells show the blue or deep blue appearance,but white blood cells and other fungus are not stained,it is very easy to differentiate Cryptococcus neoformans from white blood cells and other fungus.The stability of Evans blue staining reagent is very good,the Evans blue dyeing effect of Cryptococcus neoformans is better than Alcian blue stain and ink stain,Cryptococcus neoformans can be counted in counting plate under microscope by Evans blue staining and it can not be easily counted by Alcian blue stain because it is easy to agglutinate,but it can not be counted by ink stain.Conclusion： Rapid identification of Cryptococcus neoformans by Evans blue staining technology need simple facilities.It′s fast and sensitive to detect,well specificity and stability,better for using in all hospitals.
出处
《中国卫生检验杂志》
CAS
2010年第9期2197-2199,共3页
Chinese Journal of Health Laboratory Technology
基金
浙江省医药卫生科学研究基金(2009B162)
关键词
新型隐球菌
伊文思蓝
墨汁染色
快速鉴定
Cryptococcus neoformans（CN）
Evans Blue
Ink Stain
Rapid Identification
