摘要
目的建立8种细菌性脑膜炎多重聚合酶链反应(Multiplex-polymerase Chain Reaction M-PCR)检测方法。方法针对脑膜炎奈瑟菌、流行性感冒嗜血杆菌、肺炎链球菌、金黄色葡萄球菌、单核细胞增生性李斯特菌、猪链球菌、结核杆菌、新生隐球菌的种特异性基因,通过优化单一PCR扩增体系及扩增程序,建立M-PCR方法检测8种导致细菌性脑膜炎的病原体,检测M-PCR体系的灵敏度。结果初步建立了检测8种导致细菌性脑膜炎病原体的M-PCR扩增方法,能够同时检测多种导致细菌性脑膜炎的病原。结论M-PCR方法较常规的分离培养鉴定方法具有较高的灵敏度和检测快速的特点,适合临床疑似细菌性脑膜炎感染病例的检测及筛查,提高临床细菌性感染脑膜炎病例的诊断阳性率。
Objective To develop a rapid method for detecting 8 pathogens which were highly related to bacterial meningitis by multiplex polymerase chain reaction. Method By optimizing the reaction condition and amplification program of single pair polymerase chain reaction, the multiplex pairs polymerase chain reactions （M-PCR）was developed to identify eight pathogens simuhaneously including Neisseria Meningitidis, Haemophilus Influenzae, Streptococcus Pneumoniae, Cryptococcus Neoformans, Staphylococcus Aureus, Listerisa Monocytogene, Streptococcus Suis and Mycobacterium Tuberculosis. Meanwhile, The sensitivity of M-PCR assay was also studied. Results M-PCR methods for detecting 8 pathogens which could cause bacterial meningitis have been established. M-PCR showed specific, sensitive and more rapid than conventional culturing method. Conclusion This multiplex polymerase chain reaction method can be used for diagnosis and scanning of suspicious bacterial meningitis cases in order to improve the diagnostic positive rate of bacterial meningitis cases.
出处
《中国疫苗和免疫》
CAS
2010年第1期47-51,共5页
Chinese Journal of Vaccines and Immunization
关键词
细菌性脑膜炎
多重聚合酶链反应
Bacterial meningitis
Muhiplex-polymerase chain reaction
