摘要
目的建立一种于正常群体中快速、准确、成本低廉的检测龋易感人群的方法。方法随机选取3～4岁无龋儿童125名为研究对象,联合运用聚合酶链反应(PCR)和gtfB寡核苷酸探针杂交法来检测研究对象唾液中的变异链球菌。追踪观察1年,进行临床复查,将初查与复查的结果进行比较,通过前瞻性研究对此方法作出评价。结果单独采用PCR法检测龋易感人群时,其预测灵敏度、预测特异度、预测可信度分别为56.4%、44.2%、48.3%;联合采用PCR和探针杂交法检测龋易感人群时,上述3个指标均有提高,分别为69.2%、46.8%、54.3%。探针杂交试验阳性和阴性者都检查到有龋病发生,但阳性患龋者的龋失补牙数(dmft)为2.15±0.86,龋病患病率为23.28%,而阴性患龋者的dmft为1.58±0.51,龋病患病率为10.34%;二者有统计学差异(P<0.05),阳性组的dmft和患龋率均大于阴性组。结论本研究所用的gtfB寡核苷酸探针在检测龋易感人群方面具有潜在优势,预测灵敏度较高,但预测特异度和可信度不高,仍需进一步研究。
Objective To establish a quick,exact and inexpensive method to detect caries susceptibility in children.Methods 125 caries free children,aged 3-4 years,were randomly sampled.A combination of polymerase chain reaction（PCR） and gtfB oligonucleotide probe hybridization method was used to detect Streptococcus mutans in saliva.The participants were followed up for a year and the clinical examination results were compared with the laboratory results.The perspective study was used to evaluate the detecting approach.Results When the combination of PCR and hybridization method was used to detect caries susceptibility of the sample,the predictive sensitivity,predictive specificity and predictive reliability were all increased to 69.2%,46.8% and 54.3%,respectively as compared to only PCR,which were 56.4%,44.2% and 48.3%,respectively.The samples with both the positive and negative results of hybridization detection had caries clinically,but the dmft index and prevalence were higher in the positive（dmft was 2.15±0.86,and the prevalence was 23.28%） than in the negative（dmft was 1.58±0.51,and the prevalence was 10.34%） which was statistically significant（P〈0.05）.Conclusion The probe was found to be quite potential in detecting caries susceptibility,but the predictive specificity and predictive reliability values were not significant.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2010年第4期378-381,386,共5页
West China Journal of Stomatology
基金
陕西省科技攻关基金资助项目[2004K12-G4(3)]
关键词
变异链球菌
龋活性试验
寡核苷酸探针
Streptococcus mutans
caries activity test
oligonucleotide probe
