摘要
目的 采用寡核苷酸探针研究龈下菌斑中 3种牙周致病菌的分布。方法 利用 3种寡核苷酸探针对6 0例慢性牙周炎患者 6 0个患病位点、10例健康人的 10个健康对照位点龈下菌斑中的牙龈卟啉单胞菌、福赛类杆菌、牙密螺旋体进行检测。结果 牙周炎位点龈下菌斑中的牙龈卟啉单胞菌、福赛类杆菌、牙密螺旋体的检出率分别为 91 6 7%,90 0 0 %和 95 6 7%,明显高于健康对照位点 ;有 83 33%的牙周炎位点同时检出 3种致病菌 ,3种细菌检出情况为两两正相关 (P <0 0 1)。结论 牙龈卟啉单胞菌、福赛类杆菌、牙密螺旋体在慢性牙周炎患者龈下菌斑中的检出率很高 ,它们间可能存在相互协同致病作用。
Objective To observe the prevalence of Porphyromonas gingivalis(Pg),Treponema denticola(Td) and Bacterides forsythus(Bf) subgingivally in diseased sites of chronic periodontitis (CP) patients. Methods Samples of subgingival plaque were detected by 3 kinds of oligonucleotide DNA probe from 60 sites of CP patients and 10 healthy sites of 10 healthy people.Results The positive rates of Pg, Bf and Td in patients were 91.67%, 90.00% and 95.67% respectively; Pg, Bf and Td were detected simultaneously in 83.33% patients and Pg, Bf and Td were found to be related with each other(P<0.01).Conclusion Pg,Bf and Td were prominent periodontopathic bacteria and related each other, might exist in complexes in subgingival plaque and coaggretate together.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2003年第4期287-288,310,共3页
West China Journal of Stomatology
关键词
寡核苷酸探针
龈下菌斑
致病菌
检测
牙周炎
periodontitis
Porphyromonas gingivalis
Treponema denticola
Bacterides forsythus
oligonucleotide DNA probe
