摘要
目的 :探讨来自不同龋敏感者的变形链球菌 (血清型c)临床分离株的粘附能力。方法 :采用液体闪烁计测法测量 3H TDR标记的变形链球菌临床分离株对唾液包被羟磷灰石 (SHA)的粘附。结果 :同一个体所带不同基因型菌株对SHA粘附量差异较大 ;高龋组个体定植的粘附能力强的菌株所占比例显著高于无龋组。结论 :高龋组变形链球菌 (血清型c)临床菌株的高致龋力与其携带有粘附能力强的菌株密切相关。
Objective:In this study, authors used saliva coated hydroxyapatite to simulate tooth surface in oral cavity to evaluate adherence of different Streptococcus mutans isolated from caries free and active persons to saliva coated hydroxyapatite.Methods: Streptococcus mutans isolated from people with different carious experiences were divided into two groups: the caried free group(DMFS=0) including 19 persons and the caries active group (DMFS≥6) including 40 persons. Totally 105 genotype Streptococcus mutans strains were obtains, including 22 strains from the caries free group, and 83 strains from the caries active group. The adhering properties of Streptococcus mutans (serotype c) strains were investigated, and different adhering properties were compared between the caries free group and the caries active group in order to find the correlationship between carious experiences and adhering properties of Streptococcus mutans. Saliva coated hydroxyapatite was used to simulated tooth surface in oral cavity. Streptococci mutans were cultured in TPY liquid medium containing \+3 H TDR in the same radioactive concentration. After being cultured in an anaerobic incubator for 18 hours, Streptococci mutans were radiolabelled by \+3 H TDR. Saliva coated hydroxyapatite and bacterial liquid(BSA=0 52) were mixed for 90 minutes. Samples were assayed by using liquid scintillation counter, and binding abilities of strains were evaluated by the count per minute(cpm). Results: Binding abilities of strains were significantly different in two groups, Caries active people harbored more Streptococci mutans with high binding abilities(cpm>2000) than caries free people did(P<0 05). The binding abilities to saliva coated hydroxyapatite were different between strains of differing genotypes isolated from the same person. Each person always harbored one or two strains with high binding abilities(cpm>2000) in the caries active group.Conclusion: ①Different genotypes have different adhering properties.②In caries active group, different genotype strains have synergic effects, and adhesion of high binding ability strains cause caries susceptibility.③The differences in binding properties of isolates might relate to differences in carious experiences.\;
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2000年第6期416-418,共3页
West China Journal of Stomatology
关键词
变形链球菌
龋敏感性
致龋性能
龋齿
S.mutans
carious experiences
adherence
saliva-coated hydroxyapatite
