摘要
利用反转录（ＲＴ）及ＰＣＲ扩增并测定了中国猪瘟兔化弱毒（Ｃ－株）兔组织毒、Ｃ－株细胞疫苗毒和近期（１９９７～１９９８年）甘肃省７个地区的１０个流行野毒株的Ｅ２基因核酸序列。通过序列比较和系统发生关系分析发现：Ｃ－株兔组织毒、Ｃ－株细胞毒和中国５０～６０年代流行的石门强毒株同属于组群１（Ｇｒｏｕｐ１）；近期猪瘟流行毒株同属于组群２（Ｇｒｏｕｐ２）的两个不同的亚组群（Ｓｕｂｇｒｏｕｐ２．１和２．２），其流行毒株与疫苗毒株有较大的差异（Ｅ２全基因核苷酸序列同源性８２．２％～８４．３％），表明猪瘟流行毒株向远离疫苗毒株的方向演变。
The E2 gene nucleotide sequences of the Chinese hog cholera lapinized virus (C
strain), C-strain cell vaccine virus and ten strains of recent prevalent field hog cholera viruses
(HCV) collected from the seven distrincts, Gansu province, in 1997 to 1998, were amplified and
determined by a reverse transcription and polymerase chain reaction technique. And by the
comparisons of the sequences and the analysis of the phylogenetic relationship it was found
that the C strain lapinized virus, the C strain cell vaccine virus and the virulent Shimen strain
that had been epidemic in China in 1950' to 1960' all belong to the Group 1; the recent prevalent
field strains of HCV are divided into the various subgroups of the Group 2 (the subgroups 2.1
and 2.2), which showed that obvious differences between the C strain and the recent prevalent
strains of HCV exist (the nucleotide homologies of the both were 82.2% to 84.3%). And it
indicated that the prevalent strains of HCV have been evolving to the direction far of
出处
《中国兽医科技》
CSCD
1999年第6期3-7,共5页
Chinese Journal of Veterinary Science and Technology
基金
甘肃省自然基金
国家攀登计划资助
关键词
猪瘟病毒
流行
遗传发生关系
Hog Cholera VirusPrevalencePhylogenetic relationship
