摘要
利用反转录聚合酶链式反应 (RT PCR)及测序技术 ,测定 2 0 0 2年分离于我国甘肃、湖南、海南、青海等省的 9株猪瘟野毒的E2基因序列 ,并与C 株疫苗毒进行同源性比较 ,绘制CSFV系统发生树。结果表明 ,近期分离于我国的 9株野毒与C 株核苷酸序列间的同源性在80 .7%～ 99.1 %,氨基酸同源性在 89.3%～98.9%。9株野毒分属于 2个不同的组群 ,其中 5株与我国经典的石门强毒和C 株疫苗毒同属于Subgroups1 .1 ,另 4株属于与C 株有较大差异的Subgroup2 .1。
The E2 gene nucleotide sequences of nine field strains of Classical swine fever virus (CSFV) isolated from Gansu, Hunan, Hainan and Qinghai province of China in 2002 were amplified by reverse transcription (RT) and nested polymerase chain reaction (nPCR), and then, the amplified fragments were cloned and sequenced. The E2 nucleotide sequences and the deduced amino acid sequences of nine strains and C-strain were compared and analyzed by DNAStar software. The further study on genetic relationship was done by phylogenic tree constructed on the base of analyzing the dataset of E2 gene with computer software. The result revealed that the homology of the nucleotide sequences between nine field strains and the C-strain were from 80.7 to (99.1) percent and the deduced amino acid sequences were from 89.3 to 98.9 percent and they were classified to two different Groups in the phylogenetic tree, five of the nine recent field strains belong to Subgroup 1.1 which contain Shimen strain and C-strain, and the other four strains belong to Subgroup 2.1.
出处
《动物医学进展》
CSCD
2004年第6期69-71,共3页
Progress In Veterinary Medicine
基金
国家 973项目"畜禽重大疫病病原大分子比较与功能研究"
社会公益项目"畜禽重大疫病监测与控制系统研究"
关键词
中国
猪瘟
分子流行病学
动态分析
Classical swine fever virus
E2 gene
molecular epidemiology
