摘要
利用反转录－PCR方法扩增了吉林省猪瘟病毒（HCV）两个野毒株gp55基因的主要保护性抗原编码区，并将其克隆到pGEM－T载体中，然后用Sanger双脱氧法测定了其核苷酸序列，并推导了其氨基酸序列。将测定的这两个HCV野毒株的部分序列（350bp）与国内外已知的HCV序列进行比较，结果表明：这两个野毒株的核苷酸序列的同源性为94．9％，氨基酸序列同源性为97．4％，与1985～1992年意大利中部分离4个野毒株的同源性明显高于其它HCV毒株，核苷酸同源性分别为97．2％～98．3％和94.0％～949％，氨基酸同源性分别为98．3％～991％和97.4％～98.3％，而与我国的HCV标准强毒株即石门株的核苷酸同源性仅分别为83.1％和83.1％，氨基酸同源性仅分别为90.6％和91.4％。因此认为吉林省这两个野毒株与意大利中部的4个野毒株具有密切的关系，而与石门株很可能来源不同。
One pair of oligonucleotide primers were designed to amplify the region of hog cholera virus (HCV)genome, which corresponds to a 588 bp portion encoding the major protective antigen re-gion, a part of gp55 protein. The product was amplified from two virus isolates (strains CC and JL)which had been responsible for two hog cholera outbreaks in Jilin province in the northeast of China. The cDNA products were cloned into pGEM - T vector. Nucleotide sequencing was per-formed by Sanger's method. A part of the obtained two HCV (CC and JL) sequences, 350 bp,were compared with other HCV strains. This allowed confidently assigning the two virus isolates and 4 virus isolates from the central reglons of Italy to a subgroup. But the Chinese standard viru-lent strain Shimen was assigned to another subgroup.
出处
《中国病毒学》
CSCD
1997年第4期354-359,共6页
Virologica Sinica
基金
国家自然科学基金
国家攀登计划B类项目
