摘要
从大庆油田油藏采出水中成功筛选获得一株表面活性剂产生菌,编号为SQ6,该菌为短杆状革兰氏阴性菌。16S rRNA基因序列比对结果显示,SQ6与铜绿假单胞菌(Pseudomonas aeruginosa)序列相似性达到99.9%;生理生化鉴定结果进一步表明,SQ6符合假单胞菌属特征。以葡萄糖为碳源,24h内SQ6发酵液表面张力(ST)从57.0mN·m-1降至25.6mN·m-1。SQ6发酵液在高温(121℃,30min)、高盐(NaCl浓度为12%)和广泛pH条件下保持较稳定的表面活性,其临界胶束浓度(CMC)为50mg·L-1。薄层层析结果显示,SQ6发酵液表面活性成分主要为糖脂类。
A short rod shaped Gram negative surfactant-producing bacterium designated as SQ6 was isolated from the produced water from Daqing oilfield. The physiological and biochemical test showed that the SQ6 had the characteristics of Pseudomonas, and the 16S rRNA gene sequence analysis further indicated that the SQ6 had a similarity of 99.9% to Pseudomonas aeruginosa. Growing on the mineral salts medium containing 2% glucose, the SQ6 decreased the surface tension of SQ6 zymotic fluid from 57.0 to 25.6 mN·m^-1 within 24 hours. Under the conditions of high temperatures （121 °C for 30 min）, high salinity （12% NaCl）, and a wide range of pH, the SQ6 zymotic fluid could maintain a relatively stable surface activity, and its critical micelle concentration was 50 mg·L^-1. Thin layer chromatography （TLC） indicated that glycolipid was the main surface-active component presented in SQ6 zymotic fluid.
出处
《生态学杂志》
CAS
CSCD
北大核心
2010年第8期1618-1623,共6页
Chinese Journal of Ecology
基金
中国科学院知识创新工程项目(06LYQY3001)
大庆油田有限责任公司作研究资助项目
