摘要
将带1－2个芽眼的唐菖蒲球茎切块接种到附加1．0mg／LBA的MS基本培养基上可诱导休眠芽萌动、无菌芽转移至附加3．0mg／LBA的培养基上可分化产生丛生芽。丛生芽的继代增殖宜采用附加1．5mg／LBA的培养基生根培养，以MS+NAA0．1－0．5mg／L或MS＋IBA1．5mg／L效果最佳。试管苗移栽存活率可达50％左右。
The dormant buds from gladiola corms were induced to sprout on MS medium supplemented with 1. 0mg/L BA. The germ free buds transferred onto the medium containing 3. 0mg/L BA could different late and produce rosette buds. During the subculture, the BA concentration should be reduced to 1. 5mg/L. The suitable rooting-media were MS + NAA 0. 1-0 5mg/L and MS + IBA 1. 5mg/L.
关键词
唐菖蒲
茎芽
离体培养
快速繁殖
Gladiola
corm-bud
in vitro culture
rapid propagation
