摘要
目的比较靶向Bcl-2、Bcl-xl、Mcl-1、Bcl-w、A1反义核酸(antisense oligodeoxynucleotide,ASO)对消化系统肿瘤细胞(肝癌HepG2细胞、胃癌MGC-803细胞、结肠癌Lovo细胞)的增殖抑制作用和致凋亡作用的差异。方法分别合成靶向Bcl-2、Bcl-xl、Mcl-1、Bcl-w、A1的反义核酸和随机序列反义核酸(randomolig odeoxynucleotide,RODN),采用脂质体Li-pofectamineTM 2000转染细胞,WST法检测相同浓度的5种ASOs对肝癌HepG2细胞、胃癌MGC-803细胞、结肠癌Lovo细胞的增殖抑制作用和致凋亡作用。结果 Bcl-2、Bcl-xl、Mcl-1、Bcl-w、A1等5种ASOs中,不论是对细胞增殖抑制还是致凋亡作用,均以Bcl-xlASO、Mcl-1ASO的作用效果较好。结论在Bcl-2、Bcl-xl、Mcl-1、Bcl-w、A1等5种ASOs中,Bcl-xlASO、Mcl-1ASO可明显抑制消化系肿瘤细胞增殖,诱导细胞凋亡。
Aim To exam the difference in proliferatory inhibition effects of Bcl-2,Bcl-xl,Mcl-1,Bcl-w and A1of Bcl-2 proteins family members antisense oligodeoxynucleotides on gastrointestinal cancer cells （ HepG2, Lovo,MGC-803） . Methods The antisense oligodeoxynucleotide targeting for Bcl-2,Bcl-xl,Mcl-1,Bcl-w and A1 and a random oligodeoxynucleotide （ RODN） were synthesized and transfected into gastrointestinal cancer cells （ HepG2,Lovo,MGC-803 ） using LipofectamineTM2000 for 48 hours,cell proliferation inhibition was determined by WST method and cell apoptotic level examined by flow cytometry. Results Antisense oligodeoxynucleotide targeting Bcl-xl and Mcl-1 gene could suppress the growth of gastrointestinal cancer of HepG2,Lovo,MGC-803 cells and induce apoptosis of these cells significantly （ P〈0. 05） . The other antisense oligodeoxynucleotide targeting Bcl-2,Bcl-w and A1 showed an indistinctively effect on inhibiting cell proliferation and inducing cell apoptosis. Conclusion Antisense oligodeoxynucleotide targeting Bcl-xl and Mcl-1 can inhibit proliferation and induce apoptosis of gastrointestinal cancer cells of HepG2,Lovo,MGC-803 cells in vitro.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2010年第8期1093-1098,共6页
Chinese Pharmacological Bulletin
基金
广东省科技计划资助项目(No2009B080701051)
广东省医学科研资助基金(NoA2009348)
