摘要
应用鸡胚培养、电镜观察、血清学试验及病理组织学检查，分离与鉴定了来自河南省鸡传染性法氏囊病（ＩＢＤ）免疫鸡群Ｈｅ１、Ｈｅ２、和Ｈｅ３三株ＩＢＤＶ毒株。鸡胚传代试验表明，Ｈｅ１和Ｈｅ２株连续传代到第４代出现规律性死亡和ＩＢＤ病变，而Ｈｅ３株在第１代时鸡胚即全部死亡，且出现ＩＢＤ典型鸡胚病变。Ｈｅ１、Ｈｅ２和Ｈｅ３毒株对易感鸡致病力有一定的差异。病死率分别为２０％、１０％和４０％，多数为弱毒株。免疫保护试验证明，用常规的ＩＢＤＶ血清Ｉ型病毒株Ｂ２疫苗对Ｈｅ１、Ｈｅ２和Ｈｅ３毒株的免疫保护试验，与对照组相比保护率分别为０％、０％和１０％。未死亡扑杀的免疫试验鸡法氏囊有不同程度的病理组织学病变，表明Ｉ型毒株疫苗对ＩＢＤ基本上无免疫保护作用。所分离的毒株可能是ＩＢＤＶ的变异株。
Chickenembryoculture,electronicmicroscopyserologicaltest,andpathotissuelogicalexaminationwereperformedtoisolateandidentifythreestrainsofavianinfectiousbursaldiseasevirus(IBDV)He1,He2,andHe3isolatedfromserologicalICommercialIBDVaccineimmunedchickensinHenanprovinceChickenembryopassageindicatedthatregulardeathandIBDpathologicalchangeshappenedatthefourthgenerationofconsecutivepassagesforHe1andHe2strainswhileforHe3strain,allthechickenembryosdiedwithtypicalpathologicalchangesatthefirstgenerationTherewerepathogerucitydifferencestosusceptiblechickensamongHe1,He2andHe3strains,lethalityof20%,10% and40%respectivelyImmunetestshowedthatIBDVserumI commercialvaccinenearlylindnoprotectiontoHe1,He2andHe3withtheprotectiveratesof0%,0% and10%respetivelyTheHe1,He2andHe3aremostprobablyvariationstrainsofIBDV
出处
《中国预防兽医学报》
CAS
CSCD
1999年第3期171-174,共4页
Chinese Journal of Preventive Veterinary Medicine
