摘要
对利用IBDV地方野毒株所制备的囊毒和胚毒二价灭活油乳苗免疫效果进行了比较研究。结果表明,所制备的囊毒灭活苗免疫效果显著优于胚毒灭活苗和活疫苗,在接种后各个时期所产生的抗体效价均显著高于其它两种疫苗(P<0.01),最高AGP效价可达4.8log2;胚苗的免疫效果虽然差于囊苗,但明显优于活疫苗,当活苗免疫组鸡血清中已无可测性抗体时,胚苗组仍具有1.0log2的AGP效价。在攻毒试验中,囊苗组鸡对IBDV标准毒、两株野毒株的攻击均100%地获得了保护;胚苗组也均可获得80%的保护,这两种由地方毒株所制备的疫苗均未呈现出对不同毒株抵抗性的不同,而活苗组对不同毒株的攻击则分别只有53.3%、40%和46.7%的保护率,呈现出一定的差异性。
The efficacy of the bivalent oil-adjuvant inactivated vaccines preparated using cultures of bursal tissues and embryo ailantoic liquid of two field isolates were detected separately.The results showed that the efficacy of vaccine prepared with bursal tissue was obviously superior to those of the other two vaccines.The titres of AGP antibody were obviously higher than those of the other vaccine post-immunization（P〈0.01）,the highest AGP titre was 4.81og2.Although the efficacy of vaccine prepared with embryo allantoic liquid was not better than that of bursal vaccine,it was obviously better than that of live vaccine.Immuned chickens challenged with IBDV BC6/85 and vvlBDV isolates.100% and 80% of chickens immunized with inactivated vaccines were protectod,respectively.The resistance of two vaccines prepared with vvIBDV isolates against various IBDV had no difference,but the resistance of live vaccine against various IBDV had a little difference.
出处
《中国家禽》
北大核心
2006年第5期11-13,共3页
China Poultry
关键词
IBDV地方野毒株
囊组织
胚尿囊液
二价灭活油乳苗
IBDV isolates
bursal tissues
embryo allantoic liquid
bivalent oil-adjuvent inactivated vaccines
