摘要
基于抗坏血酸在碱性条件下与高碘酸钾和鲁米诺反应产生很强的化学发光，建立了流动注射化学发光测定抗坏血酸的新方法。在碱性条件下的氧化过程易产生过氧化物和超氧自由基，是产生化学发光的关键。该法校准曲线线性范围为６．０×１０－６～４．０×１０－２ｇ／Ｌ，检出限达８×１０－７ｇ／Ｌ，相对标准偏差小于４％（ｎ＝１１），采样频率为１２０次／ｈ。具有灵敏度高，线性范围宽，选择性好的优点，对药用维生素Ｃ中抗坏血酸含量进行测定，得到满意结果。
The chemiluminescence
reaction of ascorbic acid with potassium periodate and luminol in a alkaline solution has been
studied.A novel method with flow injection for the determination of ascorbic acid was proposed ,
and the mechanism of reaction was also discussed .The linear range of the calibration curve is
6.0×10 -6 ～4.0×10 -2 g/L,and the detection limit is 8×10 -7 g/L with a throughput of
120 samples per hour.The RSD of determination is less than 4%( n =11).The method has been
applied to the determination of ascorbic acid in pharmaceutical samples with high sensitivity
and selectivity ,and satisfactory results were obtained.
出处
《分析试验室》
CAS
CSCD
1999年第2期58-61,共4页
Chinese Journal of Analysis Laboratory
