摘要
采用单因素轮换法优化β-胡萝卜素合成的发酵条件,考察因素包括正负菌种龄、发酵周期、接种量、正负菌种接种比例、初始pH、发酵温度、装液量、摇床转速。结果表明,三孢布拉霉菌的最佳摇瓶发酵条件为:正菌种龄12h～15h、负菌种龄25h～30h、发酵周期120h、接种量20%、正负菌种接种比例2:3、初始pH6.5、发酵温度28℃、装液量50mL/250mL三角瓶、摇床转速200rpm,β-胡萝卜素产量为28.8mg/L。
The elementary fermentation conditions were obtained through a series of single factor rotation method. Based on the analysis of these results, the optimal culture conditions were confirmed by orthogonal test. The results showed that the optimum conditions are： seed age of strain （＋） 12～15h and strain （-） 25～30h、fermentation time 120h、inoculum size 20%、 the ratio of strain （＋） to strain （-）2：3、original pH6.5、culture temperature 28℃、medium volumes 50mL/250mL triangular flask、rotating speed 200 rpm. β-carotene production was 28.8mg/L.
出处
《食品与发酵科技》
CAS
2010年第4期36-40,共5页
Food and Fermentation Science & Technology
基金
湖北省自然科学基金重点项目(项目编号:2009CDA059)
关键词
三孢布拉霉
Β-胡萝卜素
发酵条件
Blakeslea trispora
β-carotene
fermentation conditions
