摘要
目的：探讨多肿瘤抑制基因１（ＭＴＳ１）对卵巢癌细胞系增殖的影响．方法：绘制细胞生长曲线图，比较卵巢癌细胞系ＨＯ－８９１０（无ｐ１６基因的表达）与８９１０－ｐ１６（转染ｐ１６基因）、８９１０－ｐｃＤＮＡ３（转染空载体ｐｃＤＮＡ３）的生长特性；采用３Ｈ－ＴｄＲ掺入实验比较３种细胞的ＤＮＡ合成情况．结果：８９１０－ｐ１６细胞在转染ｐ１６基因后其增殖特性受到抑制，ＤＮＡ的合成也随之降低，而８９１０－ｐｃＤＮＡ３与ＨＯ－８９１０间无明显差异．结论：多肿瘤抑制基因１（ＭＴＳ１）能抑制卵巢癌细胞系的增殖．
AIM: To investigate the effect of multiple tumor suppressor 1 on the proliferation of ovarian cancer cell line HO 8910. METHODS: By protracting the cell growth curve, we studied the distinctness of growth characteristics among the HO 8910 (no p 16 gene expressing), 8910 p 16 (transfected p 16 gene) and 8910 pcDNA3 (transfected the vector pcDNA3). In the meantime,we also compared the distinctness of DNA synthesis among the three kinds of cells. RESULTS: After transfected p 16 gene, the proliferation and DNA synthesis of 8910 p16 were markedly inhibited. There was no difference between the 8910 pcDNA3 and HO 8910. CONCLUSION: Multiple tumor suppressor 1 can inhibit the proliferation and DNA synthesis of human ovarian cancer line HO 8910.
出处
《第四军医大学学报》
1999年第4期351-353,共3页
Journal of the Fourth Military Medical University
