摘要
以盆栽宽叶缬草野生苗为材料,切取其嫩叶、叶柄、地下幼嫩根状茎和不定根作为外植体,探讨不同消毒方式及不同生长调节剂组合对愈伤组织诱导和不定芽分化的影响.结果表明,嫩叶和叶柄外植体的消毒方式以70%酒精浸泡30s,再用0.1%升汞浸泡6min效果较好;地下部外植体因受污染比较严重,消毒困难,死亡率为100%.叶片和叶柄均能诱导出愈伤组织,且产生具香味的毛状根.适宜的愈伤组织诱导培养基为MS+2,4-D2mg/L+6-BA0.5mg/L,以叶为外植体的诱导率较高,可达80%.愈伤组织在供试培养基中分化困难,最高分化率仅为25%,培养基为MS+6-BA4mg/L+NAA0.5mg/L.以具芽点分化的愈伤组织为材料进行石蜡切片,显微观察显示,不定芽的发生起源于愈伤组织表皮细胞的分裂,属于外起源.
Amplification protocol in vitro was developed for multiplication of Valeriana officinalis L. var. latifolia Miq. by using leaves, petiols, rhizome and roots derived from wild plants grown in pot as explants. Effects of different plant growth regulators on callus induction, adventitious bud differentiation and bud anatomy were studied. The results showed that it was optimal for the explants to be disinfected with 70% ethanol for 30 s and then soaked with 0.1% mercuric chloride for 6 min, the optimum medium for callus induction was MS＋2,4-D 2 mg/L ＋6-BA 0.5 mg/L, and callus induction rate of leaves was up to 80%. However, buds were hard to regenerate from callus, and the highest differential rate was 25% on the medium of MS＋6-BA 4 mg/L＋NAA 0.5 mg/L. Buds regenerated exogenous from callus epidermal cells.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第4期395-398,F0003,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省教育厅项目(09C877)
关键词
宽叶缬草
组织培养
愈伤组织
不定芽
Valeriana officinalis L.
tissue culture
callus
adventitious bud
