摘要
目的 评价七氟烷预处理对脂多糖（lipopolysaccharide,LPS）所致大鼠急性肺损伤（acute lung injury,ALI）的影响.方法 72只SD大鼠随机分成6组：NS组、LPS组和七氟烷预处理（S-1 h组、S-6 h组、S-12 h组、S-24 h组）.通过气管内滴注LPS建立大鼠ALI模型.大鼠在气道内给予LPS或NS后6 h处死,检测不同时间点七氟烷预处理（2.4%,30 min）对支气管肺泡灌洗液（bronchoalveolar lavage fluid,BALF）中白细胞计数、细胞因子TNF-α和IL-1β水平,肺组织髓过氧化物酶（myeloperoxidase,MPO）活性,肺血管通透性及肺组织病理学等的影响.结果 与NS组相比,LPS组肺组织损伤程度,BALF白细胞计数以及TNF-α和IL-1β水平,血管通透性和肺组织MPO活性均显著增高（P〈0.01）.给予LPS前1 h和24 h七氟烷预处理能降低肺组织MPO活性,BALF中IL-1β水平及白细胞计数（P〈0.01）,而S-6h组和S-12 h组与IPS组相比无明显差别.七氟烷预处理均能够降低肺血管通透性和BALF中TNF-α水平（P〈0.01）,且以S-1 h和S-24h组为显著（P〈0.01）.提示七氟烷预处理对LPS所致肺损伤具有早期和延时的保护作用.结论 气道内给予LPS1 h和24 h前七氟烷预处理对LPS致大鼠ALI具有保护作用.
Objective To investigate the effects of sevoflurane pretreatment on Lipopolysaccharide （LPS）-induced acute lung injury （ALI） in rats. Methods Seventy -two Sprague -Dawley rats were randomly divided into 6 groups： NS group, LPS group and sevoflurane pretreatment （S-l h group, S-6 h group, S-12 h group and S-24 h group）. The rat model of ALI was established by intratracheal instillation of LPS. Animals were sacrificed at 6 h after LPS or NS administration. Leukocyte count, concentration of TNF-α and IL-β in bronchoalveolar lavage fluid （BALF）; pulmonary capillary permeability, myeloperoxidase （MPO） activity of lung tissue; lung histological changes were compared in rats with or without sevoflurane pretreatment （2.4% inspired for 30 min） at different times before LPS instillation. Results Compared with the NS group,severe injury of lung tissues and increase in leukocyte count in BALF, Production of TNF-α and IL-1β in BALF, pulmonary capillary permeability and MPO activity in the lung were significantly increased in rats treated with LPS （P〈0.01）. MPO activity, leukocyte count and production of IL-1βin BALF were reduced when sevoflurane was given 1 or 24 h before but not at 6 or 12 h before LPS instillation（P〈0.01）. Sevoflurane pretreatment also attenuated pulmonary capillary permeability and production of TNF-α in BALF （P〈0.01）. Pulmonary capillary permeability and concentration of TNF-α in S-l h and S-24 h group was lower than S-6 h and S-12 h group （P〈0.01）. Sevoflurane pretreatment was effective at 1 h and 24 h suggesting sevoflurane has early and late protection against LPS-induced lung injury. Conclusion Sevoflurane pretreatment has protective effects against acute lung injury when given 1 or 12 h before LPS instillation.
出处
《国际麻醉学与复苏杂志》
CAS
2010年第3期199-202,共4页
International Journal of Anesthesiology and Resuscitation
关键词
急性肺损伤
七氟烷
脂多糖
细胞因子
Acute lung injury
Sevoflurane
Lipopolysaccharides
Cytokines
