摘要
用动力学方法详细研究了Ｈｇ２＋离子对漆树漆酶催化活性的影响．结果表明，Ｈｇ２＋离子对漆酶的催化活性有明显的活化作用．这种作用是瞬时完成的，具有竞争性特征，活化剂常数Ｋａ．为７．６９×１０－５ｍｏｌ·ｄｍ－３．在一定条件下，Ｈｇ２＋离子可使漆酶的催化活性增加一倍以上．据我们所知，这是首次报道Ｈｇ２＋离子对酶有活化作用，同时也是漆酶被活化的首次报道．
Kinetic experiments were designed to examine the effe
cts of Hg2+ion on the catalytic activity of Rhus vernicifera laccase.T
he result was that Hg2+ion had obvious activation on the catalytic activit
y of laccase.The action was completed immediately and had the character of compe
titive type,the activation constant Ks=7.69×10-5mol·dm-3.The
enzyme activity of laccase activated by Hg2+ion was twice than that of na
tive laccase under certain conditions.To our knowledge this is the first example
that Hg2+ion can activate the enzyme activity,and also of the fact that t
he enzymatic activity of laccase can be activated.
基金
广西自然科学基金
关键词
漆树
漆酶
汞离子
催化活性
活化作用
rhus vernicifera laccase
mercury(Ⅱ)
competitive
activation
kinetics
