摘要
以5,6-二溴-2,3-二氰基氢醌(DDBQH2)为底物,在pH值4.4和30±0.2℃条件下,用分光光度法考察了Ag+离子对漆树漆酶催化活性的影响。结果表明,Ag+离子对DDBQH2的漆酶催化氧化反应有明显的抑制作用,这种作用具有罕见的反竞争性特征,抑制剂常数Ki=(2.1±0.5)×10-5moldm-3。
Kinetic experiments were designed to examine the effects of Ag + ion on the oxidation of DDBQH 2(5,6 dibromo 2,3 dicyanohydroquinone) catalyzed by Rhus vernicifera laccase. The result was that Ag + ion could uncompetitively inhibit the catalytic activity of laccase at pH4 4 and 30±0 2℃,the inhibition constant K i =(2 1±0 5)×10 -5 mol·dm -3
出处
《广西科学》
CAS
1999年第2期109-110,114,共3页
Guangxi Sciences
基金
广西自然科学基金