摘要
目的有研究提示基质金属蛋白酶(martix metalloproteinase,MMP)及其抑制物金属蛋白酶组织抑制剂(tissue inhibitor of metalloproteinase,TIMP)、转化生长因子(transforming growth factor,TGF)-β1在细胞外基质(extracellular matrix,ECM)降解过程中起着重要的作用,但MMP、TIMP和TGF-β1在胎膜早破(premature rupture of membrane,PROM)中的作用机制尚不明了。文中的研究目的为探讨PROM患者胎盘胎膜组织中MMP-9、TIMP-1和TGF-β1表达变化及意义。方法采用免疫组织化学SP法和Western blot印迹杂交法分别检测足月未临产择期剖宫、足月自然临产阴道分娩和足月PROM各28例孕妇分娩后的胎盘胎膜组织中MMP-9,TIMP-1和TGF-β1表达变化。结果免疫组化结果显示:①未临产组胎膜组织中MMP-9表达强度最低(P<0.05),临产组次之(P<0.05),PROM组最高(P<0.05),胎盘中MMP-9表达趋势亦同;②TIMP-1和TGF-β1表达趋势与MMP-9相反,未临产组胎膜组织中TIMP-1和TGF-β1表达强度最高(P<0.05),临产组次之(P<0.05),PROM组最低(P<0.05),胎盘中TIMP-1和TGF-β1亦然。Western blot结果显示:①未临产组胎膜组织中MMP-9表达量最低(P<0.05),临产组次之(P<0.05),PROM组最高(P<0.05),胎盘中MMP-9表达趋势亦同;②未临产组胎膜组织中TIMP-1和TGF-β1表达量最高(P<0.05),临产组次之(P<0.05),PROM组最低(P<0.05),胎盘中TIMP-1和TGF-β1亦然。胎盘和胎膜上TGF-β1表达水平与TIMP-1表达呈正相关,与MMP-9表达呈负相关。结论胎盘胎膜上TGF-β1表达降低,可通过调节MMP-9和TIMP-1的表达引起MMP-9/TIMP-1之间的比例失衡,使胶原降解加速,胎膜强度与韧性下降,从而导致PROM的发生。
Objective Matrix metalloproteinase （MMP）,the tissue inhibitor of metalloproteinase （TIMP）,and the transforming growth factor （TGF）-β1 play the important role in the degradation of extracellular matrix （ECM）,but the roles of MMP-9,TIMP-1 and TGF-β1 in premature rupture of membranes （PROM） are not yet clear.The aim of this study was to explore the expressions of MMP-9,TIMP-1 and TGF-β1 in the placenta and fetal membrane of PROM patients and their clinical significance.Methods We detected by immunohistochemistry and Western blot the expressions of MMP-9,TIMP-1 and TGF-β1 in the placenta tissues and fetal membranes from term pregnancy women,including 28 normal cases not in labor （group A）,28 normal cases in labor （group B）,and another 28 PROM patients in labor （group C）.Results The results of immunohistochemistry and Western blot showed the expression of MMP-9 in the placenta and fetal membrane to be the lowest in group A,and the highest in group C （P 〈0.05）,while the expressions of TIMP-1 and TGF-β1 were just the opposite,the highest in A and the lowest in C （P 〈0.05）.The expression of TGF-β1 was correlated positively with TIMP-1 but negatively with MMP-9.Conclusion The down-regulated TGF-β1 may cause imbalance of MMP-9 and TIMP-1 in the fetal membrane and placenta,and result in PROM.
出处
《医学研究生学报》
CAS
2010年第7期700-705,共6页
Journal of Medical Postgraduates
基金
2008年江苏省六大人才高峰基金(DG216D5019)
关键词
胎膜早破
基质金属蛋白酶-9
金属蛋白酶组织抑制剂-1
转化生长因子-Β1
Premature rupture of membranes
Matrix metalloproteinases-9
Tissue inhibitor of metalloproteinase-1
Transforming growth factor-β1
