摘要
目的：探讨食管癌多部位DNA含量与临床及病理特性之间的关系。方法：流式细胞光度术检测100例食管癌手术切除新鲜标本的癌、癌旁和断端组织的DNA含量。结果：癌组织DNA非整倍体（AN）、DNA指数（DI）和细胞增殖活性指数（PI）都显著高于癌旁和断端（P＜0．05）；食管癌AN与肿瘤细胞分化和局部淋巴结转移密切相关（P＜0．01）。在癌体与癌旁或断端组织间存在DNA倍体异质性（14．8％）；切距＜5cm的断端组织AN和PI显著高于切距≥5组（P＜0．05）。结论：食管癌多部位流式细胞DNA含量的分析在揭示肿瘤恶性生物学行为、指导临床治疗和术后随访具有重要的意义。
: PurPose: To determine the correlation of DNA content with clinicopathologic features of esophageal carcinoma. Methods: DNA content was determined by Flow cytometry on freshly multiple specimens from cancerous tissues,pericancerous and incision margin epithelium fmm 100 patients. Results: DNA aneuploidy (AN), DNA index (DI) and the proliferation index (PI) of cancerous group were significantly higher than those of pericancerous or incision margin group (P <0. 05). AN was found to closely correlate with both tumor cell differetiation and the frequency Of lymph node metastasis (P < 0. 01). of the 27 cases with diploid tumor, 4(14. 8% ) show DNA ploid heterogeneity between cancerous tissue and pericancerous or incision margin tissues. AN and PI in cut distance from tumor margin to incision margin <5 cm group were significantly higher than those in ≥5 cm group (P <0.05). Conclusions: Flow cytometric DNA content analysis on multiple locations of esophageal careinoma is a valued method in determining the highly malignant Potential and detecting clinical therapy and Postoperative surveillance.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1999年第1期69-72,共4页
Chinese Journal of Cancer
关键词
食管肿瘤
DNA含量
Esophageal carcinoma
Flow cytometry
DNA content
Cell cycle
Heterogeneity
