摘要
为研究食管鳞状细胞癌瘤体内DNA倍体异质性及其意义，采用多点取材法，应用流式细胞术对80例食管鳞状细胞癌DNA倍体进行了研究。结果：（1）DNA指数范围为0．77～1．74，DNA异倍体的检出率为88．8％（71／80例）；（2）80例中，（4．5％38例）瘤体内表现为倍体异质现象；（3）DNA倍体异质性与肿瘤的浸润深度、淋巴结转移及患者预后有关，而与组织学分级及瘤体大小无关。结果揭示，用DNA倍体异质性估价肿瘤的生物学特性和预后可能比单点取材异倍体更为准确。
In order to investigate the intratumoral DNA ploid heterogeneity in esophageal squamous cell carcinoma and its significance, nuclear DNA pIoidy of 80 cases of squamous cell carcinoma of the esophagus were determined for multiple samples obtained from the same tumor, uslng a flow cytometry (FCM) technique. The results showed that: (1) DNA indeces ranged from 7. 7~ 1.74, and the incidence of DNA aneuploidy was 88. 8% (71/80) in this series; (2) of 80 cases, (47. 5 % 38 cases )showed intratumoral heterogeneity in DNA ploidy (3) the heterogeneity in DNA ploidy was related to the extent of wall penetration by tumor, the incidnce of lymph node metastasis and patient's prognosis,not to histological grades and size of tumor. The results from this study indicated that DNA ploidy heterogeuneity may be a exact indicator in reflecting the biological characteristics of tumor and patient's prognosis than DNA aneuploidy from single site sampling.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1996年第3期159-161,共3页
Chinese Journal of Pathology
基金
河南省教委青年资金
