摘要
目的：探讨人参皂甙Ｒｂ１、Ｒｇ１及维生素Ｅ对氧化损伤后的牛视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ，ＲＰＥ）细胞的抗氧化作用。方法：建立牛ＲＰＥ细胞氧化损伤的模型，并分为正常对照组（１ｍｌＤＭＥＭ培养液），次黄嘌呤（ｈｙｐｏｘａｎｔｈｉｎｅ，ＨＸ）／黄嘌呤氧化酶（ｘａｎｔｈｉｎｅｏｘｉｄａｓｅ，ＸＯ）损伤组（ＨＸ／ＸＯ组），Ｒｂ１组，Ｒｇ１组和维生素Ｅ组。利用硫代巴比妥酸比色法，检测各组ＲＰＥ细胞培养上清液中脂质过氧化（ｌｉｐｉｄｐｅｒｏｘｉｄａｔｉｏｎ，ＬＰＯ）产物丙二醛（ｍａｌｏｎｄｉ－ａｌｄｅｈｙｄｅ，ＭＤＡ）含量。结果：ＨＸ／ＸＯ组ＭＤＡ与正常对照组相比有显著性差异（Ｐ＜０．０５），Ｒｂ１、Ｒｇ１和维生素Ｅ组中两种药物浓度均能有效地降低ＭＤＡ的生成（Ｐ＜０．０５）。
OBJECTIVE: To investigate effect on ginsenoside Rb1, Rg1, and vitamin E in the prevention from lipid peroxidation of bovine retinal pigment epithelium cells.METHODS: Cultured bovine RPE cells were erected, were divided into control group, hypoxanthine ( HX )/ xanthine oxidase ( XO ) Rg1 and Rg1 group ( HX/XO ), and Vitamin E group. The thiobarbituric acid (TBA) assay was performed to detect the content of malondialdehyde (MDA) in the culture supernatant fluid of RPE cells. RESULTS: There was statistical significant between the control group and HX/XO group in contents of MDA ( P <0 05). The content of MDA was decreased significantly in the Rb1 group, Rg1 group, and vitamin E group when two concentrations were applied ( P <0 05). CONCLUSIONS: Ginsenoside Rb1, Rg1 and vitamin E can inhabit the increase of MDA from lipid peroxidation in RPE cells.
出处
《中国中医眼科杂志》
1999年第1期3-6,共4页
China Journal of Chinese Ophthalmology
基金
北京市自然科学基金
关键词
视网膜色素上皮
脂质过氧化
人参皂甙
维生素E
retinal pigment epithelium cells
lipid peroxidation ( LPD )
malondialdehyde ( MDA )
ginsenoside
vitamin E
