摘要
目的：观察鼻咽癌细胞株CNE－1和CNE－2裸鼠移植瘤组织中肿瘤细胞凋亡的形态学特征，并探讨p53，bc1－2和bax在瘤细胞凋亡中所起的作用。方法：CNE－1和CNE－2鼻咽癌细胞株接种于裸鼠并在裸鼠体内连续传3代。在HE和TUNEL染色片中观察细胞株和各代移植瘤组织中癌细胞的凋亡；应用免疫组化法检测p53，bc1－2和bax基因的表达；抽提细胞株及各代移植瘤组织的DNA，以PCR－SSCP法检测p53基因第5～8外显子的改变。结果：在CNE－1和CNE－2移植瘤组织中有相当数量的瘤细胞发生凋亡，可见较大面积的“皱缩性坏死”区和众多的凋亡小体。PCR－SSCP显示p53基因第8外显子有改变，在细胞涂片和各代移植瘤组织中都有p53蛋白的积聚。3代移植瘤组织均有bax过表达，却无bc1－2表达。结论：CNE－1和CNE－2裸鼠移植瘤组织中肿瘤细胞死亡的主要途径是凋亡，表现为大量的“皱缩性坏死”和凋亡小体的形成；这种凋亡可能是非p53依赖性，由bax所介导的。
Objective:TO identify the morphological characteristics of neoplastic cell apoptosis developed in nude mice transplants of nasopharyngeal careinoma (Nab)cell lines, CNE － 1 and CNE － 2 and to investigate the roles of p53, hcf － 2 and bax playing in the process of apoptosis. Methods: CNE:－ 1 and CNE － 2 cell lines were inoculated and passed in nude face for 3 generations. The apoptosis was detected on H&E and TUNEL staining slides. The expression of p53, bc1－ 2 and bax were observed using immunohistoc hemistry. ps 3 gene alteration was assayed in cell lines and transplant ti ssues by PCR SSCP. Results:A considerable number of neoplastic cells underwent apoptosis in CNE － 1 and CNE － 2 nude mice transplant tissues. The 'shrinkage necrosis' and apoptotic bodies were the main appearances of apoptosis. The p53 alteration was detected in exon & by PCR － SSCP and p53 protein accumulation observed in the cell smears and nude mice transplant tissue sections. All the transplant tissue sections of 3 passages showed bc1 － 2 negativity and bax overexpression. ConclusionS: (1 )The neoplastic cells in the nude mice transplants of CNE－ 1 and CNE － 2 cell lines underwent death mainly via apoptosis;(2) 'Shrinkage necrosis' and apoptotic bodies were the main morphological appearances of apoptosis; (3) The apoptosis developed in nude mice transplants of CNE － 1 and CNE－ 2 NPC cell lines is highly probable through a p53 － independent and bax － mediated pathway.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1999年第2期172-175,共4页
Chinese Journal of Cancer
基金
国家自然科学基金!39730200-Ⅱ
关键词
鼻咽肿瘤
裸鼠
移植瘤
细胞凋亡
Nasopharyngeal neoplasms
Nude mice transplant
Apoptosis
