摘要
目的：采用ＨＰＬＣ测定心宁胶囊中葛根素含量，以控制该制剂的质量。方法：用十八烷基键合硅胶柱分离葛根素，以甲醇水磷酸（２８０∶７２０∶０．１５）为流动相，检测波长２５０ｎｍ。结果：葛根素峰与其它组分峰的分离度大于１，理论塔板数以葛根素峰计算为８３１０；线性范围０．２～２μｇ（ｒ＝０．９９９９）；平均回收率９９．４％，ＲＳＤ＝２．４７％（ｎ＝５）。结论：本法简便、快速。
OBJECTIVE:To domminate the quality of Xinning capsule,the contents of Puerarin in Xinning capsule was determined by HPLC.METHOD:Puerarin was separated on Spherisorb C 18 column and detected at 250nm,by using methanol water phosphoric acid (280∶720∶0.15)as mobile phase.RESULTS:The resolution was greater than 1. The number of theoretical plates calculaled for Puerarin peak was 8310;The standard curve was linear over the range of 0.2～2μg with the correllation coefficient 0.9999.The average recovery was 99.4% with RSD =2.47%( n =7).CONCLUSION:The method is simple and fast,and can be used for quantitative analysis of Xinning capsule.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
1999年第3期187-189,共3页
Chinese Pharmaceutical Journal
