摘要
目的：寻找新的肿瘤细胞凋亡诱导剂。方法：选用茶的主要活性成分茶多酚，采用噻唑蓝（ＭＴＴ）还原法，ＤＮＡ凝胶电泳以及透射电镜技术，在体外观察了茶多酚对人急性早幼粒白血病细胞（ＨＬ－６０）凋亡的影响。结果：被２５０ｍｇ／Ｌ茶多酚作用５ｈ后ＨＬ－６０细胞进行凝胶电泳呈现出典型的ＤＮＡ条带，透射电镜下看到凋亡小体，茶多酚对ＨＬ－６０细胞凋亡的诱导活性平行于它的细胞杀伤作用。结论：在体外培养条件下。
AIM To study anticarcinogenic mechanism of polyphenolic compounds in tea.METHODS: The apoptosis inducing effects of tea polyphenols (TP), the main active component purified from tea, were observed on the human promyelocytic leukemic cell (HL-60) by thiazolyl blue tetrazolium bromide reduction test, DNA agarose gel electrophoresis and transmission electronic microscopy technique.RESULTS:After treating HL-60 cells with TP in 250mg·L 1 for 5h, DNA extracted from HL-60 cells showed a typical internucleosomal DNA degradation, i.e., DNA ladder and apoptosis vehicles were observed. The effect of TP was showed to parallel with its cytotoxic activity in HL-60 cells.CONCLUSION:The mechanism of antitumor action of TP is related to its apoptosis inducing activity.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第3期264-267,共4页
Chinese Journal of Pathophysiology
