摘要
应用３２Ｐ后标记法检测人羊膜上皮细胞（ＦＬ）经不同浓度茶多酚（２５μｇ／ｍｌ，５０μｇ／ｍｌ和１００μｇ／ｍｌ，终浓度，下同）和β－萘黄酮（２×ｌ０－５ｍｏｌ／Ｌ）所形成的Ｂ（ａ）Ｐ－ＤＮＡ加成物，观察茶多酚化学预防作用。结果表明，茶多酚不仅可以抑制β－萘黄酮对ＦＬ细胞细胞色素Ｐ４５０同功酶的活性的诱导，减少Ｂ（ａ）Ｐ－ＤＮＡ加成物的形成；也可抑制经β－萘黄酮诱导后的ＦＬ细胞形成Ｂ（ａ）Ｐ－ＤＮＡ加成物，而且该抑制效应与茶多酚剂量一致。茶多酚的化学预防作用可能与其影响细胞色素Ｐ４５０同功酶的表达调节以及抑制其活性有关。
With32Ppostlabeling method ,a study was performed to detect the formation of B(a)P-DNA adducts in cultured human amnion FL cells treated by different combinations ofgreen tea polyphenols(GTPP, 25μg/ml,50μg/ml and l00μg/ml )andβ-naphthoflaone(β-NF,Z×10-8mol/L ).The results showed that GTPP could inhibit either theβ-naph- thoflavone inducing expression of cytochrome P450 in FL cells,or the activity of the cy-tochrome P4s0 isozyme inβ-NF induced cells,thus reduce the formation of B(a)P-DNAadducts in these cells.
出处
《癌变·畸变·突变》
CAS
CSCD
1995年第2期65-68,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
